Human H3N2 Influenza Viruses Isolated from 1968 To 2012 Show Varying Preference for Receptor Substructures with No Apparent Consequences for Disease or Spread

被引:93
作者
Gulati, Shelly [1 ]
Smith, David F. [2 ]
Cummings, Richard D. [2 ]
Couch, Robert B. [3 ]
Griesemer, Sara B. [4 ]
George, Kirsten St. [4 ]
Webster, Robert G. [5 ]
Air, Gillian M. [1 ]
机构
[1] Univ Oklahoma, Hlth Sci Ctr, Dept Biochem & Mol Biol, Oklahoma City, OK 73190 USA
[2] Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA
[3] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[4] Wadsworth Ctr, New York State Dept Hlth, Albany, NY USA
[5] St Jude Childrens Res Hosp, Memphis, TN 38105 USA
来源
PLOS ONE | 2013年 / 8卷 / 06期
关键词
AMINO-ACID CHANGE; BINDING SPECIFICITY; SIALIC-ACID; HEMAGGLUTININ; CELLS; OLIGOSACCHARIDES; NEURAMINIDASE; AGGLUTINATION; RECOGNITION; SEQUENCES;
D O I
10.1371/journal.pone.0066325
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
It is generally accepted that human influenza viruses bind glycans containing sialic acid linked alpha 2-6 to the next sugar, that avian influenza viruses bind glycans containing the alpha 2-3 linkage, and that mutations that change the binding specificity might change the host tropism. We noted that human H3N2 viruses showed dramatic differences in their binding specificity, and so we embarked on a study of representative human H3N2 influenza viruses, isolated from 1968 to 2012, that had been isolated and minimally passaged only in mammalian cells, never in eggs. The 45 viruses were grown in MDCK cells, purified, fluorescently labeled and screened on the Consortium for Functional Glycomics Glycan Array. Viruses isolated in the same season have similar binding specificity profiles but the profiles show marked year-to-year variation. None of the 610 glycans on the array (166 sialylated glycans) bound to all viruses; the closest was Neu5Ac alpha 2-6(Gal beta 1-4GlcNAc)(3) in either a linear or biantennary form, that bound 42 of the 45 viruses. The earliest human H3N2 viruses preferentially bound short, branched sialylated glycans while recent viruses bind better to long polylactosamine chains terminating in sialic acid. Viruses isolated in 1996, 2006, 2010 and 2012 bind glycans with alpha 2-3 linked sialic acid; for 2006, 2010 and 2012 viruses this binding was inhibited by oseltamivir, indicating binding of alpha 2-3 sialylated glycans by neuraminidase. More significantly, oseltamivir inhibited virus entry of 2010 and 2012 viruses into MDCK cells. All of these viruses were representative of epidemic strains that spread around the world, so all could infect and transmit between humans with high efficiency. We conclude that the year-to-year variation in receptor binding specificity is a consequence of amino acid sequence changes driven by antigenic drift, and that viruses with quite different binding specificity and avidity are equally fit to infect and transmit in the human population.
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