Peripheral antioxidant enzyme activities and selenium in elderly subjects and in dementia of Alzheimer's type - Place of the extracellular glutathione peroxidase

被引:112
作者
CeballosPicot, I
MeradBoudia, M
Nicole, A
Thevenin, M
Hellier, G
Legrain, S
Berr, C
机构
[1] HOP LA PITIE SALPETRIERE, INSERM U360, F-75651 PARIS, FRANCE
[2] HOP NECKER ENFANTS MALAD, DEPT BIOCHEM B, PARIS, FRANCE
[3] HOP NECKER ENFANTS MALAD, DEPT BIOCHEM A, PARIS, FRANCE
[4] INSERM U169, VILLEJUIF, FRANCE
[5] HOP PAUL BROUSSE, SERV GERONTOL CLIN & SOINS PALLIATIFS, VILLEJUIF, FRANCE
关键词
Alzheimer's disease; age; oxidative stress; superoxide dismutase; glutathione peroxidase; peripheral markers; free radicals;
D O I
10.1016/0891-5849(95)02058-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Defenses against free radical damage were determined in red blood cells and plasma from 40 patients with dementia of the Alzheimer-type (DAT) and 34 aged control subjects with normal cognitive function. No crude significant difference in erythrocyte copper-zinc superoxide dismutase (E-CuZnSOD), seleno-dependent glutathione peroxidase (E-GSH-Px), glutathione reductase (E-GSSG-RD) activities, and selenium (Se) concentration was found between DAT cases and control subjects. The peroxidation products evaluated in plasma by the thiobarbituric-reactive material (TEARS) were at the same level in the DAT group as compared to controls. In the DAT group, plasma GSH-Px (P-GSH-Px) activity and plasma Se (P-Se) were negatively correlated with age (r = -0.58; p < 0.001 and r = -0.63; p < 0.001 respectively). Moreover, erythrocyte GSH-Px activity and Se were also negatively correlated with age (r = -0.40; p < 0.01 and r = -0.46; p < 0.01, respectively). No significant correlation with age was observed in the controls. When controlling for age, a significant increase for P-GSH-Px activity and P-Se was observed in DAT patients as compared to controls. These significant differences mostly appeared in DAT subjects under 80 years. Some correlations were only observed in the DAT group such as P-GSH-Px and E-GSH-Px (r = +0.68; p < 0.001); P-GSH-Px and E-Se (r = +0.79; p < 0.001). Correlations between P-GSH-Px and P-Se, E-GSH Pr and P-Se, and P-Se with E-Se are greater in the DAT group (r = +0.84; p < 0.001; r +0.76; p < 0.001 and r 0.75; p < 0.001) than in the control group (r = 0.54, pI < 0.01; r = 0.43, p < 0.01 and r = +0.34, p < 0.05 respectively). The fact that first-a significant increase in P-GSH-Px and P-Se, second-some modifications in the relationships between antioxidant parameters, and third-age-dependent decreases of glutathione-peroxidase activities and their cofactor, appeared only in the DAT group suggest that DAT is associated with an oxidative stress due to an imbalance between reactive oxygen species and the peripheral antioxidant opposing forces.
引用
收藏
页码:579 / 587
页数:9
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