Differential localization of non-muscle myosin II isoforms and phosphorylated regulatory light chains in human MRC-5 fibroblasts

被引：59

作者：

Saitoh, T

Takemura, S

Ueda, K

Hosoya, H

Nagayama, M

Haga, H

Kawabata, K

Yamagishi, A

Takahashi, M ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Sci, Div Chem, Sapporo, Hokkaido 0600810, Japan

[2] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan

[3] Hokkaido Univ, Grad Sch Sci, Div Phys, Sapporo, Hokkaido 0600810, Japan

[4] Hiroshima Univ, Grad Sch Sci, Dept Biol Sci, Higashihiroshima 7398526, Japan

来源：

FEBS LETTERS | 2001年 / 509卷 / 03期

关键词：

myosin II heavy chain isoform; regulatory light chain; phosphorylation; localization; cell migration; stress fiber;

D O I：

10.1016/S0014-5793(01)03186-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the localization of non-muscle myosin II isoforms and mono- (at serine 19) and diphosphorylated (at serine 19 and threonine 18) regulatory light chains (RLCs) in motile and non-motile MRC-5 fibroblasts. In migrating cells, myosin IIA localized to the lamella and throughout the posterior region. Myosin IIB colocalized with myosin HA to the posterior region except at the very end. Diphosphorylated RLCs were detected in the restricted region where myosin HA was enriched. In non-motile cells, myosin IIA was enriched in peripheral stress fibers with diphosphorylated RLCs, but myosin IIB was not. Our results suggest that myosin HA may be highly activated by diphosphorylation of RLCs and primarily involved in cell migration. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：365 / 369

页数：5

共 42 条

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