Construction of Escherichia coli Bifidobacterium longum shuttle vector transforming B-longum 105-A and 108-A

被引：65

作者：

Matsumura, H

Takeuchi, A

Kano, Y

机构：

[1] Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto, 607, 1 Shichono-cho, Misasagi, Yamashina-ku

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 07期

关键词：

Bifidobacterium longum; Escherichia coli; shuttle vector pBLES100; transformation by electroporation; spectinomycin adenyltransferase AAD(9);

D O I：

10.1271/bbb.61.1211

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A shuttle vector, pBLES100, was constructed by cloning a Bifidobacterium longum plasmid and a gene encoding spectinomycin adenyltransferase AAD(9) from Enterococcus faecalis into the Escherichia coli vector pBR322. Stable transformants with this plasmid were obtained with an efficiency of 2.2 x 10(4) transformants/mu g DNA or 6.9 x 10(-5) transformants/cell/mu g DNA under the optimal conditions of 10.0 kV/cm, 200 Omega, and 25 mu F, using B. longum 105-A harvested at late log phase of growth.

引用

页码：1211 / 1212

页数：2

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