Flow cytometric analysis of the multiple drug resistance phenotype

被引：19

作者：

Krishan, A

Sauerteig, A

Andritsch, I

Wellham, L

机构：

[1] Department of Radiation Oncology, University of Miami Medical School, Sylvester Compreh. Cancer Center, Miami, FL

[2] Experimental Therapeutics Division, University of Miami Medical School, Miami, FL 33101

来源：

LEUKEMIA | 1997年 / 11卷 / 07期

关键词：

efflux; anthracyclines; P-glycoprotein; MDR; flow cytometry; fluorescence;

D O I：

10.1038/sj.leu.2400684

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Laser flow cytometry is increasingly used for quantitation of cellular fluorescent drug retention, effect of efflux blockers and for expression of drug resistance related cellular surface markers. Several intrinsic and extrinsic factors can affect the results obtained from drug retention functional assays and lead to artifacts. In the present study, we have used a panel of well-characterized parental and drug resistant cell lines, fluorochromes and efflux blockers to identify the possible sources of artefacts in flow cytometric analysis of the multiple drug resistance phenotype.

引用

页码：1138 / 1146

页数：9

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