Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins

被引：24

作者：

Ageta, H

Kato, A

Fukazawa, Y

Inokuchi, K

Sugiyama, H ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Sci, Dept Biol, Higashi Ku, Fukuoka 8128581, Japan

[2] Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan

[3] Mitsubishi Kasei Inst Life Sci, Tokyo 1948511, Japan

来源：

MOLECULAR BRAIN RESEARCH | 2001年 / 97卷 / 02期

关键词：

Vesl-1S/Homer-1a; proteasome; MG132; lactacystin; synaptic plasticity;

D O I：

10.1016/S0169-328X(01)00303-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The Vesl-1S/Homer-1a proteins are upregulated during seizure and long-term potentiation, but are rapidly degraded by ubiquitin-proteasome systems under normal conditions. We examined the distribution of Vesl-IS proteins in cultured hippocampal neurons. Application of proteasome inhibitors caused accumulation of Vesl-IS immunoreactivity in the neurons which showed a punctate distribution in the cortical regions of the cells, and these puncta. were found to be juxtaposed with synaptophysin, a presynaptic, synapse-specific protein. These results suggest that Ves1-1S protein is synaptically targeted. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：186 / 189

页数：4

相关论文

共 9 条

[1] Regulation of the level of vesl-1S/homer-1a proteins by ubiquitin-proteasome proteolytic systems [J].

Ageta, H ;

Kato, A ;

Hatakeyama, S ;

Nakayama, K ;

Isojima, Y ;

Sugiyama, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (19) :15893-15897

[2] Homer: A protein that selectively binds metabotropic glutamate receptors [J].

Brakeman, PR ;

Lanahan, AA ;

OBrien, R ;

Roche, K ;

Barnes, CA ;

Huganir, RL ;

Worley, PF .

NATURE, 1997, 386 (6622) :284-288

[3] vesl, a gene encoding VASP/Ena family related protein, is upregulated during seizure, long-term potentiation and synaptogenesis [J].

Kato, A ;

Ozawa, F ;

Saitoh, Y ;

Hirai, K ;

Inokuchi, K .

FEBS LETTERS, 1997, 412 (01) :183-189

[4] Phorbol esters promote postsynaptic accumulation of Vesl-1S/Homer-1a protein [J].

Kato, A ;

Fukuda, T ;

Fukazawa, Y ;

Isojima, Y ;

Fujitani, K ;

Inokuchi, K ;

Sugiyama, H .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2001, 13 (07) :1292-1302

[5] Novel members of the Vesl/Homer family of PDZ proteins that bind metabotropic glutamate receptors [J].

Kato, A ;

Ozawa, F ;

Saitoh, Y ;

Fukazawa, Y ;

Sugiyama, H ;

Inokuchi, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (37) :23969-23975

[6] Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin [J].

Naisbitt, S ;

Kim, E ;

Tu, JC ;

Xiao, B ;

Sala, C ;

Valtschanoff, J ;

Weinberg, RJ ;

Worley, PF ;

Sheng, M .

NEURON, 1999, 23 (03) :569-582

[7] Isolation of PSD-Zip45, a novel Homer/vesl family protein containing leucine zipper motifs, from rat brain [J].

Sun, J ;

Tadokoro, S ;

Imanaka, T ;

Murakami, SD ;

Nakamura, M ;

Kashiwada, K ;

Ko, J ;

Nishida, W ;

Sobue, K .

FEBS LETTERS, 1998, 437 (03) :304-308

[8] Coupling of mGluR/Homer and PSD-95 complexes by the shank family of postsynaptic density proteins [J].

Tu, JC ;

Xiao, B ;

Naisbitt, S ;

Yuan, JP ;

Petralia, RS ;

Brakeman, P ;

Doan, A ;

Aakalu, VK ;

Lanahan, AA ;

Sheng, M ;

Worley, PF .

NEURON, 1999, 23 (03) :583-592

[9] Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of Homer-related, synaptic proteins [J].

Xiao, B ;

Tu, JC ;

Petralia, RS ;

Yuan, JP ;

Doan, A ;

Breder, CD ;

Ruggiero, A ;

Lanahan, AA ;

Wenthold, RJ ;

Worley, PF .

NEURON, 1998, 21 (04) :707-716