Stability-indicating methods for the determination of doxazosin mezylate and celecoxib

Two stability-indicating methods were developed for the determination of doxazosin mesylate (I) and celecoxib (II) in the presence of their degradation products. The first method depends on the use of first derivative spectrophotometry (D-1) at 256, 269 nm for (I) and (II), respectively. This method determines (I) and (II) in concentration ranges of 0.8-12 and 1- 20 mug ml(-1) with mean percentage accuracies of 99.21 +/- 0.88 and 99.59 +/- 1.67 % for (I) and (II), respectively. The second method depends on the quantitative densitometric evaluation of thin-layer chromatography of (I) and (II) in the presence of their degradation products without any interference. Methylisobutyl ketone-glacial acetic acid-water (20:10:10) was used as a mobile phase for (I) and cyclollexane-dichloromethane-diethyleainine (50:40:10) for (II). The chrornatograms were scanned at 248 and 253 nm for (I) and (II), respectively. This method determines (I) and (II) in concentration ranges of 1- 4 mug per spot for both drugs with mean percentage accuracies of 100. 19 0.95 and 99.91 1.95% for (I) and (II), respectively. The suggested methods were used to determine doxazosin mesylate and celecoxib in bulk-powder, laboratory-prepared mixtures and pharmaceutical dosage forms (cardura tablet and celebrex capsule). The results obtained by applying the proposed methods were statistically analysed and compared with those obtained by the reported methods. (C) 2002 Published by Elsevier Science B.V.