Flavonoid composition of three genotypes of dry bean (Phaseolus vulgaris) differing in seedcoat color

被引:44
作者
Beninger, CW
Hosfield, GL [1 ]
Bassett, MJ
机构
[1] Michigan State Univ, USDA ARS, Sugarbeet & Bean Res, Dept Crop & Soil Sci, E Lansing, MI 48824 USA
[2] Univ Florida, Inst Food & Agr Sci, Dept Hort Sci, Gainesville, FL 32611 USA
关键词
kaempferol; flavonol glycoside; astragalin; tannins; genetics;
D O I
10.21273/JASHS.124.5.514
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Three dry bean (Phaseolucs vulgaris L.) genotypes differing in seedcoat color, mineral brown (PCDJGB nu), yellow brown (PCDJGb nu), and pale greenish yellow (PCDJgb nu), were analyzed phytochemically. Kaempferol 3-O-beta-D-glucoside (astragalin) was isolated and identified by nuclear magnetic resonance spectroscopy from all three genotypes, and was the main flavonoid monomer present. Flavonoid polymers (condensed tannins) were detected by thin layer chromatography, but anthocyanins were not detected in the three genotypes. High pressure liquid chromatography analyses indicated that astragalin was present at similar concentrations in pale greenish yellow and mineral brown genotypes, but was significantly lower in yellow brown. Presently, we do not know the functions of the G and B color genes, although the presence of astragalin in the three genotypes studied indicates these genes do not appear to act in a qualitative manner with regard to astragalin production, but may control the amount of astragalin present. Subtle differences in color between these genotypes may be due to the amount and type of tannins which have secondarily polymerized with phenolics and flavonoid monomers.
引用
收藏
页码:514 / 518
页数:5
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