Immune defects in 28-kDa proteasome activator γ-deficient mice

被引:97
作者
Barton, LF
Runnels, HA
Schell, TD
Cho, YJ
Gibbons, R
Tevethia, SS
Deepe, GS
Monaco, JJ
机构
[1] Univ Cincinnati, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45267 USA
[2] Univ Cincinnati, Howard Hughes Med Inst, Cincinnati, OH 45267 USA
[3] Univ Cincinnati, Div Infect Dis, Dept Internal Med, Cincinnati, OH 45267 USA
[4] Penn State Univ, Milton S Hershey Coll Med, Dept Microbiol & Immunol, Hershey, PA 17033 USA
[5] NIAID, Viral Immunol Sect, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.4049/jimmunol.172.6.3948
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Protein complexes of the 28-kDa proteasome activator (PA28) family activate the proteasome and may alter proteasome cleavage specificity. Initial investigations have demonstrated a role for the IFN-gamma-inducible PA28alpha/beta complex in Ag processing. Although the noninducible and predominantly nuclear PA28gamma complex has been implicated in affecting proteasome-dependent signaling pathways, such as control of the mitotic cell cycle, there is no previous evidence demonstrating a role for this structure in Ag processing. We therefore generated PA28gamma-deficient mice and investigated their immune function. PA28gamma(-/-) mice display a slight reduction in CD8(+) T cell numbers and do not effectively clear a pulmonary fungal infection. However, T cell responses in two viral infection models appear normal in both magnitude and the hierarchy of antigenic epitopes recognized. We conclude that PA28gamma(-/-) mice, like PA28alpha(-/-)/beta(-/-) mice, are deficient in the processing of only specific Ags.
引用
收藏
页码:3948 / 3954
页数:7
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