The modification of screen-printed carbon electrodes with amino group and its application to construct a H2O2 biosensor

被引：15

作者：

Gao, QA ^{[1
]}

Yang, F ^{[1
]}

Ma, Y ^{[1
]}

Yang, XR ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

来源：

ELECTROANALYSIS | 2004年 / 16卷 / 09期

关键词：

screen-printed carbon electrodes; hydrogen peroxide biosensor; thionine; horseradish peroxidase; cyclic voltammetry; impedance spectroscopy;

D O I：

10.1002/elan.200302876

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrooxidation of thionine on screen-printed carbon electrode gives rise to the modification of the surface with amino groups for the covalent immobilization of enzymes such as horseradish peroxidase (HRP). The biosensor was constructed using multilayer enzymes which covalently immobilized onto the surface of amino groups modified screen-printed carbon electrode using glutaraldehyde as a bifunctional reagent. The multilayer assemble of HRP has been characterized with the cyclic voltammetry and the faradaic impedance spectroscopy. The H2O2 biosensor exhibited a fast response (2 s) and low detection limit (0.5 muM).

引用

页码：730 / 735

页数：6

共 27 条

[1] Configurations used in the design of screen-printed enzymatic biosensors.: A review [J].