Rate-limiting steps of tyrosinase-modified electrodes for the detection of catechol

被引:84
作者
Burestedt, E
Narvaez, A
Ruzgas, T
Gorton, L
Emneus, J
Dominguez, E
MarkoVarga, G
机构
[1] LUND UNIV, DEPT ANALYT CHEM, S-22100 LUND, SWEDEN
[2] UNIV ALCALA DE HENARES, FAC PHARM, DEPT ANALYT CHEM, E-28871 ALCALA DE HENARES, SPAIN
[3] INST BIOCHEM, ENZYME CHEM LAB, VILNIUS 2600, LITHUANIA
关键词
D O I
10.1021/ac950742o
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The response currents obtained for tyrosinase-modified Teflon/graphite, carbon paste, and solid graphite electrodes in the presence of catechol are analyzed primarily using rotating disk electrode experiments. The rate-limiting steps, such as the electrochemical reduction of o-quinones and the enzymatic reduction of oxygen as well as the enzymatic oxidation of catechol, are theoretically considered and experimentally demonstrated for the different electrode configurations.
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页码:1605 / 1611
页数:7
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