Protection against oxidative damage and cell death by the natural antioxidant ergothioneine

被引:134
作者
Aruoma, OI
Spencer, JPE
Mahmood, N
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Biol, London SW7 2A2, England
[2] Kings Coll London, Pharmacol Grp, London SW3 6LX, England
[3] Kings Coll London, Ctr Bioact Screening Nat Prod, Dept Pharm, London SW3 6LX, England
关键词
ergothioneine; N-acetylcysteine; peroxynitrite; antioxidants; cell death; apoptosis; movement disorders; DNA damage;
D O I
10.1016/S0278-6915(99)00098-8
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The natural antioxidant ergothioneine (EGT) was tested for its ability to inhibit cell death caused by hydrogen peroxide (H2O2) and to inhibit DNA oxidation by peroxynitrite (ONOO-) in human neuronal hybridoma cell line (N-18-RE-105). High concentrations of EGT (5 mM) were tolerated by the N-18-RE-105 cells. N-acetylcysteine (NAC) was not well tolerated by the cells at concentrations greater than 3 mM (cell viability averaged 50%). increasing concentrations of EGT increases cell viability in the presence of NAG. EGT at concentrations up to 2 mM weakly improved cell viability in the presence of H2O2. NAC at concentrations up to 2 mM weakly decreased, but not significantly, the viability of the cells. At a higher concentration of 5 man, NAC weakly protected the neuronal cells against the H2O2-induced cell death. The protection was significantly enhanced by preincubation with EGT. Ergothioneine inhibited ONOO--induced oxidative damage in isolated calf thymus DNA and DNA in N-18-RE-105 cells. The concentration of EGT in human and mammalian tissue has been estimated to be 1-2 mM, which suggests that EGT may serve as a non-toxic thiol buffering antioxidant in vivo and may find applications in pharmaceutical preparations where oxidative stability is desired. (C) 1999 Elsevier Science Ltd All rights reserved.
引用
收藏
页码:1043 / 1053
页数:11
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