Variable-number-tandem repeat typing of mycobacterium tuberculosis isolates with low copy numbers of IS6110 by using mycobacterial interspersed repetitive units

被引:153
作者
Cowan, LS
Mosher, L
Diem, L
Massey, JP
Crawford, JT
机构
[1] Ctr Dis Control & Prevent, Div AIDS STD & TB Lab Res, Natl Ctr Infect Dis, Atlanta, GA 30333 USA
[2] Michigan Dept Community Hlth, Mol Biol Sect, Lansing, MI 48909 USA
关键词
D O I
10.1128/JCM.40.5.1592-1602.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A study set of 180 Mycobacterium tuberculosis and Mycobacterium bovis isolates having low copy numbers of IS6110 were genotyped using the recently introduced method based on the variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR). The results were compared with results of the more commonly used methods, IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping. The isolates were collected in Michigan from 1996 to 1999 as part of a project to genotype all isolates from new cases of tuberculosis in the state. Twelve MIRU loci were amplified, and the amplicons were analyzed by agarose gel electrophoresis to determine the copy number at each MIRU locus. MIRU-VNTR produced more distinct patterns (80 patterns) than did IS6110 RFLP (58 patterns.), as would be expected in this study set. Spoligo-typing identified 59 patterns. No single method defined all unique isolates, and the combination of all three typing methods generated 112 distinct patterns identitying 90 unique isolates and 90 isolates in 22 clusters. The results confirm the potential utility of MIRU-VNTR typing and show, that typing with multiple methods is required to attain maximum specificity.
引用
收藏
页码:1592 / 1602
页数:11
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