A first-generation packaging cell line for Epstein-Barr virus-derived vectors

被引：68

作者：

Delecluse, HJ

Pich, D

Hilsendegen, T

Baum, C

Hammerschmidt, W

机构：

[1] GSF, Natl Res Ctr Environm & Hlth, Inst Clin Mol Biol & Tumor Genet, Dept Gene Vectors, D-81377 Munich, Germany

[2] Heinrich Pette Inst Expt Virol & Immunol, Dept Cell & Virus Genet, D-20251 Hamburg, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 09期

关键词：

D O I：

10.1073/pnas.96.9.5188

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

On the basis of the B lymphotropic Epstein-Barr virus (EBV), we have constructed a virus-free packaging cell line that allows encapsidation of plasmids into herpesvirus particles. This cell line harbors an EBV mutant whose packaging signals have been deleted. The gene vectors, which can encompass very large, contiguous pieces of foreign DNA, carry all cis-acting elements involved in amplification and encapsidation into virus-like particles as well as those essential for extrachromosomal maintenance in the recipient cell. Although this first-generation packaging cell line suffers from unwanted recombination between the helper virus genome and gene vector DNAs, this approach opens the way to delivery and stable maintenance of any transgene in human B cells.

引用

页码：5188 / 5193

页数：6

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