Protein recognition via surface molecularly imprinted polymer nanowires

被引:241
作者
Li, Y
Yang, HH [1 ]
You, QH
Zhuang, ZX
Wang, XR
机构
[1] SOA, Inst Oceanog, Qingdao 266061, Peoples R China
[2] Wenzhou Med Coll, Wenzhou 325000, Peoples R China
关键词
D O I
10.1021/ac050802i
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this paper, we present a technique for the preparation of polymer nanowires with the protein molecule imprinted and binding sites at surface. These surface imprinting nanowires exhibit highly selective recognition for a variety of template proteins, including albumin, hemoglobin, and cytochrome c. This recognition may be through a multistep adsorption, with the specificity conferred by hydrogen bonding and shape selectivity. Due to the protein imprinted sites are located at, or close to, the surface; these imprinted nanowires have a good site accessibility toward the target protein molecules. Furthermore, the large surface area of the nanowires results in large protein molecule binding capacity of the imprinted nanowires.
引用
收藏
页码:317 / 320
页数:4
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