Acrylamide and acrylic acid determination using respiratory activity of microbial cells

The possibility of acrylamide and acrylic acid determination using respiratory activity of microbial cells of the strain Brevibacterium sp. has been investigated. The conditions to obtain specific respiratory activity (SRA) of the cells for both substrates have been examined. The substrate concentration dependences of SRA have been obtained; they exhibited linearity in the range of 0.01-0.1 g/l acrylic acid and 0.01-0.075 g/l acrylamide. The selectivity of the cell respiratory activity for various organic compounds and storage conditions of the active biomass have been studied. Acrylamide quantification in the real waste waters has been made. (C) 1996 Elsevier Science Limited