A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability

被引：239

作者：

Eirew, Peter ^{[1
]}

Stingl, John ^{[1
]}

Raouf, Afshin ^{[1
]}

Turashvili, Gulisa ^{[2
]}

Aparicio, Samuel ^{[2
,3
]}

Emerman, Joanne T. ^{[4
]}

Eaves, Connie J. ^{[1
,5
]}

机构：

[1] British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada

[2] British Columbia Canc Agcy, Mol Oncol & Breast Canc Program, Vancouver, BC V5Z 1L3, Canada

[3] Univ British Columbia, Dept Pathol & Lab Med, Vancouver, BC V6T 2B5, Canada

[4] Univ British Columbia, Dept Cellular & Physiol Sci, Vancouver, BC V6T 1Z3, Canada

[5] Univ British Columbia, Dept Med Genet, Vancouver, BC V6T 1Z3, Canada

来源：

NATURE MEDICINE | 2008年 / 14卷 / 12期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1038/nm.1791

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have demonstrated that normal mouse mammary tissue contains a rare subset of mammary stem cells. We now describe a method for detecting an analogous subpopulation in normal human mammary tissue. Dissociated cells are suspended with fibroblasts in collagen gels, which are then implanted under the kidney capsule of hormone-treated immunodeficient mice. After 2-8 weeks, the gels contain bilayered mammary epithelial structures, including luminal and myoepithelial cells, their in vitro clonogenic progenitors and cells that produce similar structures in secondary transplants. The regenerated clonogenic progenitors provide an objective indicator of input mammary stem cell activity and allow the frequency and phenotype of these human mammary stem cells to be determined by limiting-dilution analysis. This new assay procedure sets the stage for investigations of mechanisms regulating normal human mammary stem cells (and possibly stem cells in other tissues) and their relationship to human cancer stem cell populations.

引用

页码：1384 / 1389

页数：6

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