The intrinsic factor-vitamin B12 receptor, cubilin, is a high-affinity apolipoprotein A-I receptor facilitating endocytosis of high-density lipoprotein

被引:221
作者
Kozyraki, R
Fyfe, J
Kristiansen, M
Gerdes, C
Jacobsen, C
Cui, SY
Christensen, EI
Aminoff, M
de la Chapelle, A
Krahe, R
Verroust, PJ
Moestrup, SK [1 ]
机构
[1] Aarhus Univ, Dept Med Biochem, Aarhus, Denmark
[2] Michigan State Univ, Dept Microbiol, E Lansing, MI 48824 USA
[3] Aarhus Univ Hosp, Dept Med & Cardiol A, Aarhus, Denmark
[4] Aarhus Univ, Inst Anat, Dept Cell Biol, DK-8000 Aarhus C, Denmark
[5] Ohio State Univ, Div Human Canc Genet, Dept Immunol & Microbiol, Ctr Comprehens Canc, Columbus, OH USA
[6] Univ Helsinki, Folkhalsan Inst Genet, FIN-00014 Helsinki, Finland
[7] Hop Tenon, Inst Natl Sante & Rech Med, U489, Paris, France
关键词
D O I
10.1038/9504
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cubilin is the intestinal receptor for the endocytosis of intrinsic factor-vitamin B-12. However, several lines of evidence, including a high expression in kidney and yolk sac, indicate it may have additional functions. We isolated apolipoprotein A-I (apoA-I), the main protein of high-density lipoprotein (HDL), using cubilin affinity chromatography. Surface plasmon resonance analysis demonstrated a high-affinity binding of apoA-I and HDL to cubilin, and cubilin-expressing yolk sac cells showed efficient I-125-HDL endocytosis that could be inhibited by IgG antibodies against apoA-I and cubilin. The physiological relevance of the cubilin-apoA-I interaction was further emphasized by urinary apoA-I loss in some known cases of functional cubilin deficiency. Therefore, cubilin is a receptor in epithelial apoA-I/HDL metabolism.
引用
收藏
页码:656 / 661
页数:6
相关论文
共 34 条
[1]   Identification of scavenger receptor SR-BI as a high density lipoprotein receptor [J].
Acton, S ;
Rigotti, A ;
Landschulz, KT ;
Xu, SZ ;
Hobbs, HH ;
Krieger, M .
SCIENCE, 1996, 271 (5248) :518-520
[2]   Mutations in CUBN, encoding the intrinsic factor-vitamin B12 receptor, cubilin, cause hereditary megaloblastic anaemia 1 [J].
Aminoff, M ;
Carter, JE ;
Chadwick, RB ;
Johnson, C ;
Gräsbeck, R ;
Abdelaal, MA ;
Broch, H ;
Jenner, LB ;
Verroust, PJ ;
Moestrup, SK ;
de la Chapelle, A ;
Krahe, R .
NATURE GENETICS, 1999, 21 (03) :309-313
[3]   Characterization of an epithelial similar to 460-kDa protein that facilitates endocytosis of intrinsic factor-vitamin B-12 and binds receptor-associated protein [J].
Birn, H ;
Verroust, PJ ;
Nexo, E ;
Hager, H ;
Jacobsen, C ;
Christensen, EI ;
Moestrup, SK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (42) :26497-26504
[4]   A RECEPTOR-MEDIATED PATHWAY FOR CHOLESTEROL HOMEOSTASIS [J].
BROWN, MS ;
GOLDSTEIN, JL .
SCIENCE, 1986, 232 (4746) :34-47
[5]   Teratogen-mediated inhibition of target tissue response to Shh signaling [J].
Cooper, MK ;
Porter, JA ;
Young, KE ;
Beachy, PA .
SCIENCE, 1998, 280 (5369) :1603-1607
[6]  
Farese RV, 1996, J LIPID RES, V37, P347
[7]   KNOCKOUT OF THE MOUSE APOLIPOPROTEIN-B GENE RESULTS IN EMBRYONIC LETHALITY IN HOMOZYGOTES AND PROTECTION AGAINST DIET-INDUCED HYPERCHOLESTEROLEMIA IN HETEROZYGOTES [J].
FARESE, RV ;
RULAND, SL ;
FLYNN, LM ;
STOKOWSKI, RP ;
YOUNG, SG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (05) :1774-1778
[8]   Cholesterol metabolism and embryogenesis [J].
Farese, RV ;
Herz, J .
TRENDS IN GENETICS, 1998, 14 (03) :115-120
[9]  
FYFE JC, 1991, J BIOL CHEM, V266, P4489
[10]  
GLASS C, 1985, J BIOL CHEM, V260, P744