The SILVA ribosomal RNA gene database project: improved data processing and web-based tools

被引:21966
作者
Quast, Christian [1 ]
Pruesse, Elmar [1 ,2 ]
Yilmaz, Pelin [1 ]
Gerken, Jan [1 ,2 ]
Schweer, Timmy [1 ]
Yarza, Pablo [3 ]
Peplies, Joerg [3 ]
Gloeckner, Frank Oliver [1 ,2 ]
机构
[1] Max Planck Inst Marine Microbiol, Microbial Genom & Bioinformat Res Grp, D-28359 Bremen, Germany
[2] Jacobs Univ Bremen gGmbH, Sch Sci & Engn, D-28759 Bremen, Germany
[3] Ribocon GmbH, D-28359 Bremen, Germany
关键词
RESOURCE; LINEAGES;
D O I
10.1093/nar/gks1219
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
SILVA (from Latin silva, forest, http://www.arb-silva.de) is a comprehensive web resource for up to date, quality-controlled databases of aligned ribosomal RNA (rRNA) gene sequences from the Bacteria, Archaea and Eukaryota domains and supplementary online services. The referred database release 111 (July 2012) contains 3 194 778 small subunit and 288 717 large subunit rRNA gene sequences. Since the initial description of the project, substantial new features have been introduced, including advanced quality control procedures, an improved rRNA gene aligner, online tools for probe and primer evaluation and optimized browsing, searching and downloading on the website. Furthermore, the extensively curated SILVA taxonomy and the new non-redundant SILVA datasets provide an ideal reference for high-throughput classification of data from next-generation sequencing approaches.
引用
收藏
页码:D590 / D596
页数:7
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