pH-dependent channel activity of heterologously-expressed main intrinsic protein (MIP) from rat lens

Wild-type rat lens main intrinsic protein (MIP) Was heterologously expressed in the membrane of Spodoptera frugiperda (Sf21) cells using the baculovirus expression system and in mouse erythroid leukaemia cells (MEL C88). Both MEL and Sf21 cell lines expressing wild-type MIP were investigated for the conductance of ions using a whole cell patch clamp technique. An increase in conductance was seen in both expression systems, particularly on lowering the pH to 6.3. In Sf21 cells, addition of antibodies to the NPA1 box resulted in a reduction of current flow. These results suggest that MIP has pH-dependent ion channel activity, which involves the NPA1 box domain. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.