Electrochemical detection of nitric oxide in biological systems

被引:9
作者
Gow, AJ
Thom, SR
Brass, C
Ischiropoulos, H
机构
[1] UNIV PENN,SCH MED,INST ENVIRONM MED,PHILADELPHIA,PA 19104
[2] UNIV PENN,SCH MED,DEPT EMERGENCY MED,PHILADELPHIA,PA 19104
[3] UNIV PENN,SCH MED,CTR LIVER,PHILADELPHIA,PA 19104
[4] UNIV PENN,SCH MED,DEPT BIOCHEM & BIOPHYS,PHILADELPHIA,PA 19104
基金
美国国家卫生研究院;
关键词
D O I
10.1006/mchj.1996.1443
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Nitric oxide is a key regulator of diverse physiological processes that include vasoregulation, platelet and neutrophil adherence, and immune defense. The detection and quantification of nitric oxide has been accomplishd by a variety of methodologies. In this paper we desribe methodologies to detect nitric oxide in solution using an electrochemical detector and provide examples of nitric oxide detection in biological systems including data from plasma and cell suspensions. The experiments outlined show that electrochemical detection is suitable for detecting and quantifying nitric oxide within biological samples and buffers. Potential pitfalls and necessary controls are discussed. The use of electrochemical detection was also found to be useful in determining the flux of nitric oxide produced by donor species in different biological buffers. (C) 1997 Academic Press.
引用
收藏
页码:146 / 154
页数:9
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