Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus

被引：76

作者：

Kasteel, DTJ ^{[1
]}

vanderWel, NN ^{[1
]}

Jansen, KAJ ^{[1
]}

Goldbach, RW ^{[1
]}

vanLent, JWM ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT VIROL,NL-6709 PD WAGENINGEN,NETHERLANDS

来源：

JOURNAL OF GENERAL VIROLOGY | 1997年 / 78卷

关键词：

D O I：

10.1099/0022-1317-78-8-2089

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.

引用

页码：2089 / 2093

页数：5

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