Conserved requirement for a plant host cell protein in powdery mildew pathogenesis

被引:400
作者
Consonni, Chiara
Humphry, Matthew E.
Hartmann, H. Andreas
Livaja, Maren
Durner, Jorg
Westphal, Lore
Vogel, John
Lipka, Volker
Kemmerling, Birgit
Schulze-Lefert, Paul
Somerville, Shauna C.
Panstruga, Ralph
机构
[1] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany
[2] Carnegie Inst Washington, Dept Plant Biol, Stanford, CA 94305 USA
[3] GSF, Natl Res Ctr Environm & Hlth, Inst Biochem Plant Pathol, Dept Plant Immun, D-85754 Oberschleissheim, Germany
[4] Inst Plant Biochem, Dept Stress & Dev Biol, D-06120 Halle, Germany
[5] USDA, Genom & Gene Discovery Unit, Albany, CA 94710 USA
[6] Univ Tubingen, Dept Plant Biochem, D-72076 Tubingen, Germany
关键词
D O I
10.1038/ng1806
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
In the fungal phylum Ascomycota, the ability to cause disease in plants and animals has been gained and lost repeatedly during phylogenesis(1). In monocotyledonous barley, loss-of-function mlo alleles result in effective immunity against the Ascomycete Blumeria graminis f. sp. hordei, the causal agent of powdery mildew disease(2,3). However, mlo-based disease resistance has been considered a barley-specific phenomenon to date. Here, we demonstrate a conserved requirement for MLO proteins in powdery mildew pathogenesis in the dicotyledonous plant species Arabidopsis thaliana. Epistasis analysis showed that mlo resistance in A. thaliana does not involve the signaling molecules ethylene, jasmonic acid or salicylic acid, but requires a syntaxin, glycosyl hydrolase and ABC transporter(4-6). These findings imply that a common host cell entry mechanism of powdery mildew fungi evolved once and at least 200 million years ago, suggesting that within the Erysiphales ( powdery mildews) the ability to cause disease has been a stable trait throughout phylogenesis.
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页码:716 / 720
页数:5
相关论文
共 28 条
[1]   The phylogeny of plant and animal pathogens in the Ascomycota [J].
Berbee, ML .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2001, 59 (04) :165-187
[2]   Recruitment and interaction dynamics of plant penetration resistance components in a plasma membrane microdomain [J].
Bhat, RA ;
Miklis, M ;
Schmelzer, E ;
Schulze-Lefert, P ;
Panstruga, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (08) :3135-3140
[3]   The barley mlo gene: A novel control element of plant pathogen resistance [J].
Buschges, R ;
Hollricher, K ;
Panstruga, R ;
Simons, G ;
Wolter, M ;
Frijters, A ;
vanDaelen, R ;
vanderLee, T ;
Diergaarde, P ;
Groenendijk, J ;
Topsch, S ;
Vos, P ;
Salamini, F ;
Schulze-Lefert, P .
CELL, 1997, 88 (05) :695-705
[4]   SNARE-protein-mediated disease resistance at the plant cell wall [J].
Collins, NC ;
Thordal-Christensen, H ;
Lipka, V ;
Bau, S ;
Kombrink, E ;
Qiu, JL ;
Hückelhoven, R ;
Stein, M ;
Freialdenhoven, A ;
Somerville, SC ;
Schulze-Lefert, P .
NATURE, 2003, 425 (6961) :973-977
[5]   Molecular phylogeny and evolution of the plant-specific seven-transmembrane MLO family [J].
Devoto, A ;
Hartmann, HA ;
Piffanelli, P ;
Elliott, C ;
Simmons, C ;
Taramino, G ;
Goh, CS ;
Cohen, FE ;
Emerson, BC ;
Schulze-Lefert, P ;
Panstruga, R .
JOURNAL OF MOLECULAR EVOLUTION, 2003, 56 (01) :77-88
[6]   SA, JA, ethylene, and disease resistance in plants [J].
Dong, XN .
CURRENT OPINION IN PLANT BIOLOGY, 1998, 1 (04) :316-323
[7]  
Freialdenhoven A, 1996, PLANT CELL, V8, P5, DOI 10.1105/tpc.8.1.5
[8]   Sequencing of Aspergillus nidulans and comparative analysis with A-fumigatus and A-oryzae [J].
Galagan, JE ;
Calvo, SE ;
Cuomo, C ;
Ma, LJ ;
Wortman, JR ;
Batzoglou, S ;
Lee, SI ;
Bastürkmen, M ;
Spevak, CC ;
Clutterbuck, J ;
Kapitonov, V ;
Jurka, J ;
Scazzocchio, C ;
Farman, M ;
Butler, J ;
Purcell, S ;
Harris, S ;
Braus, GH ;
Draht, O ;
Busch, S ;
D'Enfert, C ;
Bouchier, C ;
Goldman, GH ;
Bell-Pedersen, D ;
Griffiths-Jones, S ;
Doonan, JH ;
Yu, J ;
Vienken, K ;
Pain, A ;
Freitag, M ;
Selker, EU ;
Archer, DB ;
Peñalva, MA ;
Oakley, BR ;
Momany, M ;
Tanaka, T ;
Kumagai, T ;
Asai, K ;
Machida, M ;
Nierman, WC ;
Denning, DW ;
Caddick, M ;
Hynes, M ;
Paoletti, M ;
Fischer, R ;
Miller, B ;
Dyer, P ;
Sachs, MS ;
Osmani, SA ;
Birren, BW .
NATURE, 2005, 438 (7071) :1105-1115
[9]   Genes controlling expression of defense responses in Arabidopsis -: 2001 status [J].
Glazebrook, J .
CURRENT OPINION IN PLANT BIOLOGY, 2001, 4 (04) :301-308
[10]   The ambivalence of the barley Mlo locus:: Mutations conferring resistance against powdery mildew (Blumeria graminis f. sp, hordei) enhance susceptibility to the rice blast fungus Magnaporthe grisea [J].
Jarosch, B ;
Kogel, KH ;
Schaffrath, U .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1999, 12 (06) :508-514