Acylated flavonol glycosides from spinach leaves (Spinacia oleracea)

From spinach leaves (cv Viroflay), five new naturally occurring flavonoids have been isolated and identified by C-13 NMR, H-1 NMR, FAB-MS, UV and hydrolytic and enzymatic procedures. The new compounds were identified as spinacetin 3-O-beta-D-glucopyranosyl(1 --> 6)-[beta-D-apiofuranosyl(1 --> 2)]-beta-D-glucopyranoside, patuletin 3-O-beta-D-(2 '' feruloylglucopyranosyl)(1 --> 6)-[beta-D-apiofuranosyl(1 --> 2)]-beta-D-glucopyranoside, spinacetin 3-O-beta-D-(2 ''-p-coumaroylglucopyranosyl) (1 --> 6)-[beta-D-apiofuranosyl(1 --> 2)]-beta-D-glucopyranoside, spinacetin 3-O-beta-D-(2 ''-feruloylglucopyranosyl)(1 --> 6)-[beta-D-apiofuranosyl(1 --> 2)]-beta-D-glucopyranoside and spinacetin 3-O-beta-D-(2 '' feruloylglucopyranosyl)(1 --> 6)-beta-D-glucopyranoside. The known compounds jaceidin 4'-glucuronide, 5,3',4'-trihydroxy-3-methoxy-6 : 7-methylenedioxyflavone 4'-glucuronide, 5,4'-dihydroxy-3,3'-dimethoxy-6: 7-methylenedioxyflavone 4'-glucuronide, patuletin 3-glucosyl(1 --> 6)-[apiosyl(1 --> 2)] glucoside and patuletin and spinacetin 3-gentiobiosides, were also detected. (C) 1997 Elsevier Science Ltd. All rights reserved.