Characterization of the biosynthetic pathway of cellulose from glucose and fructose in Acetobacter xylinum

被引：41

作者：

Tonouchi, N ^{[1
]}

Tsuchida, T ^{[1
]}

Yoshinaga, F ^{[1
]}

Beppu, T ^{[1
]}

Horinouchi, S ^{[1
]}

机构：

[1] UNIV TOKYO, DEPT BIOTECHNOL, DIV AGR & LIFE SCI, BUNKYO KU, TOKYO 113, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 08期

关键词：

Acetobacter; cellulose-producing microorganism; biosynthesis; fructose-specific phosphotransferase system;

D O I：

10.1271/bbb.60.1377

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For characterization of the biosynthetic pathway of cellulose in a cellulose-producing Acetobacter xylinum strain BPR2001, the activities of several enzymes were measured. The activity of phosphoglucose isomerase catalyzing the conversion of fructose-6-phosphate into glucose-6-phosphate was greatly increased by fructose in the medium. The UDP-glucose pyrophosphorylase activity catalyzing the synthesis of UDP-glucose was very high in strain BPR2001, consistent with the idea that this is the key enzyme in cellulose biosynthesis. Strain BPR2001 was found to have a fructose-specific phosphoenolpyruvate-dependent phosphotransferase system (PTS).

引用

页码：1377 / 1379

页数：3

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