In vitro analysis of histone acetyltransferase activity

被引：5

作者：

Benson, LJ ^{[1
]}

Annunziato, AT ^{[1
]}

机构：

[1] Boston Coll, Dept Biol, Chestnut Hill, MA 02467 USA

来源：

METHODS | 2004年 / 33卷 / 01期

关键词：

histone; acetylation; chromatin; Hat1; Hat-B;

D O I：

10.1016/j.ymeth.2003.10.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interest in historic acetylation has risen dramatically in recent years. In the following report, we present methods for the analysis of histone acetyltransferase activity in vitro. Protocols are described for radiolabeling histone proteins and N-terminal "tail" peptides, and for the analysis of acetylation by immunoblotting. Techniques for acetylating immobilized histone peptides are also described. (C) 2003 Elsevier Inc. All rights reserved.

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页码：45 / 52

页数：8

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