Discovery of specific tryptophan hydroxylase in the brain of the beetle Harmonia axyridis

被引：5

作者：

Bao, XX ^{[1
]}

Tian, XM

Hu, XH

Zhao, ZF

Qu, YT

Song, CT

机构：

[1] NE Normal Univ, Lab Insect Brain Neurobiol, Sch Life Sci, Changchun 130024, Peoples R China

[2] Jilin Univ, Coll Environm & Resources, Changchun 130012, Peoples R China

[3] NE Normal Univ, Coll Urban & Environm Sci, Changchun 130024, Peoples R China

来源：

BRAIN RESEARCH | 2006年 / 1073卷

基金：

中国国家自然科学基金;

关键词：

serotonin; tryptophan hydroxylase; Harmonia axyridis; immunohistochemistry; aromatic amino acid hydroxylase;

D O I：

10.1016/j.brainres.2005.12.041

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Rabbit anti-serotonin and mouse monoclonal anti-tryptophan hydroxylase antisera. were applied on the brain sections of the beetle Harmonia oxyridis, butterfly Childrena zenobia, moth Antheraea pernyi and ant Camponotus japonicus, using the Streptavidin-Peroxidase immunohistochemical method and Colophony-Paraffin embedded section technique. Results revealed that all the experimental insects showed notable serotonin-like immunoreactivity in the brain. However, only the brain sections of the beetle H. axyridis were strongly labeled by mouse monoclonal anti-tryptophan hydroxylase antiserum, with the distribution pattern matching that of serotonin. These results demonstrate that specific tryptophan hydroxylase may exist in the brain of the beetle and likely reflect the diversity of serotonin synthetic mechanisms as well as the evolution of aromatic amino acid hydroxylase genes. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：202 / 208

页数：7

共 19 条

[1] Phenylalanine hydroxylase participation in the synthesis of serotonin and pteridines in Drosophila melanogaster [J].

Alcaniz, S ;

Silva, FJ .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1997, 116 (03) :205-212

[2] CP embedding agent and its application to the serial section of the body wall tissue of insects [J].

Bao, XX ;

Zheng, YP ;

Zhang, HS ;

Xia, YZ ;

Song, CT .

CHINESE SCIENCE BULLETIN, 1999, 44 (11) :1021-1024

[3]

BENDER DA, 1987, P ISTRY 86 5 M INT S

[4] Substrate regulation of serotonin and dopamine synthesis in Drosophila [J].

Coleman, Chandra M. ;

Neckameyer, Wendi S. .

INVERTEBRATE NEUROSCIENCE, 2004, 5 (02) :85-96

[5] Serotonin synthesis by two distinct enzymes in Drosophila melanogaster [J].

Coleman, CM ;

Neckameyer, WS .

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 2005, 59 (01) :12-31

[6] FULL-LENGTH CDNA FOR RABBIT TRYPTOPHAN-HYDROXYLASE - FUNCTIONAL DOMAINS AND EVOLUTION OF AROMATIC AMINO-ACID HYDROXYLASES [J].

GRENETT, HE ;

LEDLEY, FD ;

REED, LL ;

WOO, SLC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) :5530-5534

[7]

HOMBERG U, 1989, CELL TISSUE RES, V258, P1, DOI 10.1007/BF00223139

[8] STRUCTURE AND FUNCTION OF THE AROMATIC AMINO-ACID HYDROXYLASES [J].

HUFTON, SE ;

JENNINGS, IG ;

COTTON, RGH .

BIOCHEMICAL JOURNAL, 1995, 311 :353-366

[9]

Monastirioti M, 1999, MICROSC RES TECHNIQ, V45, P106, DOI 10.1002/(SICI)1097-0029(19990415)45:2<106::AID-JEMT5>3.0.CO

[10]

2-3

← 1 2 →