Heavy metal-mediated activation of the rat Cn/Zn superoxide dismutase gene via a metal-responsive element

被引:50
作者
Yoo, HY [1 ]
Chang, MS [1 ]
Rho, HM [1 ]
机构
[1] Seoul Natl Univ, Dept Biol Mol, Res Ctr Cell Differentiat, Seoul 151742, South Korea
来源
MOLECULAR AND GENERAL GENETICS | 1999年 / 262卷 / 02期
关键词
SOD1; gene; metal-responsive element; induction; heavy metals;
D O I
10.1007/s004380051088
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Cu/Zn superoxide dismutase (SOD1) catalyzes the dismutation of superoxide radicals produced in the course of biological oxidations. When placed under the control of the rat SOD1 gene promoter and transfected into human HepG2 hepatoma cells, the activity of a chloramphenicol acetyltransferase reporter gene was found to increase three- to four-fold in the presence of heavy metals (cadmium, zinc and copper). Functional analysis of mutant derivatives of the SOD1 gene promoter and the use of a heterologous promoter system confirmed that the induction of the SOD1 gene by metal ions requires a metal-responsive element (MRE) located between positions -273 and -267 (GCGCGCA). It was also shown by gel mobility shift assays that an MRE binding protein is induced by the exposure of the human liver cell line HepG2 to heavy metals. These results suggest that the MRE participates in the induction of the SOD1 gene by heavy metals.
引用
收藏
页码:310 / 313
页数:4
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