Enhanced activity and enantioselectivity of Candida rugosa lipase immobilized on macroporous adsorptive resins for ibuprofen resolution

被引：44

作者：

Yu, HW

Wu, JC

Ching, CB

机构：

[1] Natl Univ Singapore, Chem & Proc Engn Ctr, Singapore 117576, Singapore

[2] Inst Chem Engn, Singapore 139959, Singapore

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 08期

关键词：

Candida rugosa; ibuprofen; immobilization; lipase; macroporous adsorptive resin; resolution;

D O I：

10.1023/B:BILE.0000023020.12576.1e

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The lipase from Candida rugosa was immobilized on three commercially available macroporous adsorptive resins for kinetic resolution of ibuprofen. One resin, CRB02, increased the enzyme activity by 50% to 0.027 g g(-1) min(-1). The deactivation constant (0.19 h(-1)) of the immobilized enzyme was half of that of the native enzyme and the enantioselectivity (E = 29.2) of the immobilized lipase was 2.2 times as much as that of the native lipase for the kinetic resolution of ibuprofen with 1-propanol in isooctane at 30 degreesC.

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页码：629 / 633

页数：5

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