Detection of California serogroup bunyaviruses in tissue culture and mosquito pools by PCR

被引：5

作者：

Campbell, WP ^{[1
]}

Huang, C ^{[1
]}

机构：

[1] NEW YORK STATE DEPT HLTH,WADSWORTH CTR LABS & RES,ALBANY,NY 12201

来源：

JOURNAL OF VIROLOGICAL METHODS | 1996年 / 57卷 / 02期

关键词：

California serogroup viruses; RT-PCR; universal primers;

D O I：

10.1016/0166-0934(95)01982-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for rapid detection of 14 California serogroup viruses using universal primer pairs. These primers are specific for the small RNA (S RNA) and middle RNA (M RNA) segments. The method has been employed to detect Jamestown Canyon (JC) virus in naturally infected mosquitoes. With this technique, it is possible to detect virus in an amount of material equivalent to as little as 0.03 mosquito.

引用

页码：175 / 179

页数：5

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