Inference of the phylogenetic position of oxymonads based on nine genes: Support for Metamonada and Excavata

被引:52
作者
Hampl, V [1 ]
Horner, DS
Dyal, P
Kulda, J
Flegr, J
Foster, PG
Embley, TM
机构
[1] Charles Univ Prague, Fac Sci, Dept Parasitol, Prague, Czech Republic
[2] Univ Milan, Dept Mol Biosci & Biotechnol, Milan, Italy
[3] Nat Hist Museum, Dept Zool, London SW7 5BD, England
关键词
phylogeny; concatenation; Excavata; Metamonada; oxymonads; Monocercomonoides;
D O I
10.1093/molbev/msi245
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Circumscribing major eukaryote groups and resolving higher order relationships between them are among the most challenging tasks facing molecular evolutionists. Recently, evidence suggesting a new supergroup (the Excavata) comprising a wide array of flagellates has been collected. This group consists of diplomonads, retortamonads, Carpediemonas, heteroloboseans, Trimastix, jakobids, and Malawimonas, all of which possess a particular type of ventral feeding groove that is proposed to be homologous. Euglenozoans, parabasalids, and oxymonads have also been associated with Excavata as their relationships to one or more core excavate taxa were demonstrated. However, the main barrier to the general acceptance of Excavata is that its existence is founded primarily on cytoskeletal similarities, without consistent support from molecular phylogenetics. In gene trees, Excavata are typically not recovered together. In this paper, we present an analysis of the phylogenetic position of oxymonads (genus Monocercomonoides) based on concatenation of eight protein sequences (alpha-tubulin, beta-tubulin, gamma-tubulin, EF-1 alpha, EF-2, cytosolic (cyt) HSP70, HSP90, and ubiquitin) and 18S rRNA. We demonstrate that the genes are in conflict regarding the position of oxymonads. Concatenation of alpha- and beta-tubulin placed oxymonads in the plant-chromist part of the tree, while the concatenation of other genes recovered a well-supported group of Metamonada (oxymonads, diplomonads, and parabasalids) that branched weakly with euglenozoans-connecting all four excavates included in the analyses and thus providing conditional support for the existence of Excavata.
引用
收藏
页码:2508 / 2518
页数:11
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