Inducible immune factors of the vector mosquito Anopheles gambiae: Biochemical purification of a defensin antibacterial peptide and molecular cloning of preprodefensin cDNA

被引:105
作者
Richman, AM
Bulet, P
Hetru, C
BarillasMury, C
Hoffmann, JA
Kafatos, FC
机构
[1] EUROPEAN MOLEC BIOL LAB,D-69117 HEIDELBERG,GERMANY
[2] INST BIOL MOLEC & CELLULAIRE,UPR 9022 CNRS,F-67084 STRASBOURG,FRANCE
关键词
Anopheles gambiae; insect defensin; malaria; vector immunity;
D O I
10.1111/j.1365-2583.1996.tb00055.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Larvae of the mosquito vector of human malaria, Anopheles gambiae, were inoculated with bacteria and extracts were biochemically fractionated by reverse-phase HPLC. Multiple induced polypeptides and antibacterial activities were observed following bacterial infection, including a member of the insect defensin family of antibacterial proteins, A cDNA encoding An, gambiae preprodefensin was isolated using PCR primers based on phylogenetically conserved sequences, The mature peptide is highly conserved, but the signal and propeptide segments are not, relative to corresponding defensin sequences of other insects, Defensin expression is induced in response to bacterial infection, in both adult and larval stages, In contrast, pupae express defensin mRNA constitutively, Defensin expression may prove a valuable molecular marker to monitor the An. gambiae host response to infection by parasitic protozoa of medical importance.
引用
收藏
页码:203 / 210
页数:8
相关论文
共 34 条
[1]  
BOMAN HG, 1981, TRENDS BIOCHEM SCI, V6, P306, DOI 10.1146/annurev.mi.41.100187.000535
[2]   2-DIMENSIONAL H-1-NMR STUDY OF RECOMBINANT INSECT DEFENSIN-A IN WATER - RESONANCE ASSIGNMENTS, SECONDARY STRUCTURE AND GLOBAL FOLDING [J].
BONMATIN, JM ;
BONNAT, JL ;
GALLET, X ;
VOVELLE, F ;
PTAK, M ;
REICHHART, JM ;
HOFFMANN, JA ;
KEPPI, E ;
LEGRAIN, M ;
ACHSTETTER, T .
JOURNAL OF BIOMOLECULAR NMR, 1992, 2 (03) :235-256
[3]  
BULET P, 1991, J BIOL CHEM, V266, P24520
[4]   A NOVEL INSECT DEFENSIN MEDIATES THE INDUCIBLE ANTIBACTERIAL ACTIVITY IN LARVAE OF THE DRAGONFLY AESCHNA-CYANEA (PALEOPTERA, ODONATA) [J].
BULET, P ;
COCIANCICH, S ;
REULAND, M ;
SAUBER, F ;
BISCHOFF, R ;
HEGY, G ;
VANDORSSELAER, A ;
HETRU, C ;
HOFFMANN, JA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 209 (03) :977-984
[5]  
CASTEELSJOSSON K, 1994, J BIOL CHEM, V269, P28569
[6]   PURIFICATION OF AN INSECT DEFENSIN FROM THE MOSQUITO, AEDES-AEGYPTI [J].
CHALK, R ;
TOWNSON, H ;
NATORI, S ;
DESMOND, H ;
HAM, PJ .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1994, 24 (04) :403-410
[7]   The inducible antibacterial peptides of the hemipteran insect Palomena prasina: Identification of a unique family of proline-rich peptides and of a novel insect defensin [J].
Chernysh, S ;
Cociancich, S ;
Briand, JP ;
Hetru, C ;
Bulet, P .
JOURNAL OF INSECT PHYSIOLOGY, 1996, 42 (01) :81-89
[8]   THE INDUCIBLE ANTIBACTERIAL PEPTIDES OF INSECTS [J].
COCIANCICH, S ;
BULET, P ;
HETRU, C ;
HOFFMANN, JA .
PARASITOLOGY TODAY, 1994, 10 (04) :132-139
[9]   PURIFICATION AND CHARACTERIZATION OF A SCORPION DEFENSIN, A 4KDA ANTIBACTERIAL PEPTIDE PRESENTING STRUCTURAL SIMILARITIES WITH INSECT DEFENSINS AND SCORPION TOXINS [J].
COCIANCICH, S ;
GOYFFON, M ;
BONTEMS, F ;
BULET, P ;
BOUET, F ;
MENEZ, A ;
HOFFMANN, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (01) :17-22
[10]  
COCIANCICH S, 1993, J BIOL CHEM, V268, P19239