Berberine-DNA complexation: New insights into the cooperative binding and energetic aspects

被引:78
作者
Bhadra, Kakali [1 ]
Maiti, Motilal [1 ]
Kumar, Gopinatha Suresh [1 ]
机构
[1] Indian Inst Chem Biol, Council Sci & Ind Res, Biophys Chem Lab, Kolkata 700032, India
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2008年 / 1780卷 / 09期
关键词
berberine; cooperative binding; non-cooperative binding; heterogeneity; calorimetry; thermodynamics;
D O I
10.1016/j.bbagen.2008.05.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The equilibrium binding of the cytotoxic plant alkaloid berberine to various DNAs and energetics of the interaction have been studied. At low ratios of bound alkaloid to base pair, the binding exhibited cooperativity to natural DNAs having almost equal Proportions of AT and GC sequences. In contrast, the binding was non-cooperative to DNAs with predominantly high AT OF GC sequences. Among the synthetic DNAs, cooperative binding was observed with poly(dA).poly(dT) and poly(dG).poly(dC) while noncooperative binding was seen with poly(dA-dT).poly(dA-dT) and poly(dG-dC).poly(dG-dC). Both cooperative and non-cooperative bindings were remarkably dependent on the salt concentration of the media. Linear plots of In K. versus [Na+] for poly(dA).poly(dT) and poly(dA-dT).poly(dA-dT) showed the release of 0.56 and 0.75 sodium ions respectively per bound alkaloid. Isothermal titration calorimetry, results revealed the binding to be exothermic and favoured by both enthalpy and entropy changes in all DNAs except the two AT polymers and AT rich DNA, where the same was predominantly entropy driven. Heat capacity values (Delta Cp degrees) of berberine binding to poly(dA).poly(dT), poly(dA-dT).poly(dA-dT), Clostridium perfringens and calf thymus DNA were -98, -140, -120 and - 110 cal/mol K respectively. This study presents new insights into the binding dependent base pair heterogeneity in DNA conformation and the first complete thermodynamic profile of berberine binding to DNAs. (c) 2008 Published by Elsevier B.V.
引用
收藏
页码:1054 / 1061
页数:8
相关论文
共 61 条
[1]   The medicinal plant goldenseal is a natural LDL-lowering agent with multiple bioactive components and new action mechanisms [J].
Abidi, Parveen ;
Chen, Wei ;
Kraemer, Fredric B. ;
Li, Hai ;
Liu, Jingwen .
JOURNAL OF LIPID RESEARCH, 2006, 47 (10) :2134-2147
[2]   Protonated structures of naturally occurring deoxyribonucleic acids and their interaction with berberine [J].
Bhadra, K ;
Kumar, GS ;
Das, S ;
Islam, MM ;
Maiti, M .
BIOORGANIC & MEDICINAL CHEMISTRY, 2005, 13 (16) :4851-4863
[3]   Molecular recognition of DNA by small molecules: AT base pair specific intercalative binding of cytotoxic plant alkaloid palmatine [J].
Bhadra, Kakah ;
Maiti, Motilal ;
Kumar, Gopinatha Suresh .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2007, 1770 (07) :1071-1080
[4]   Thermodynamics of the binding of cytotoxic protoberberine molecule coralyne to deoxyribonucleic acids [J].
Bhadra, Kakali ;
Maiti, Motilal ;
Kumar, Gopinatha Suresh .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2008, 1780 (02) :298-306
[5]  
BHAKUNI DS, 1986, ALKALOIDS, V28, P95
[6]   ENTHALPY ENTROPY COMPENSATIONS IN DRUG DNA-BINDING STUDIES [J].
BRESLAUER, KJ ;
REMETA, DP ;
CHOU, WY ;
FERRANTE, R ;
CURRY, J ;
ZAUNCZKOWSKI, D ;
SNYDER, JG ;
MARKY, LA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) :8922-8926
[7]  
Chaires JB, 1997, BIOPOLYMERS, V44, P201, DOI 10.1002/(SICI)1097-0282(1997)44:3<201::AID-BIP2>3.0.CO
[8]  
2-Z
[9]   EQUILIBRIUM STUDIES ON THE INTERACTION OF DAUNOMYCIN WITH DEOXYPOLYNUCLEOTIDES [J].
CHAIRES, JB .
BIOCHEMISTRY, 1983, 22 (18) :4204-4211
[10]  
Chaires JB, 1996, ANTI-CANCER DRUG DES, V11, P569