Adhesion of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments

被引：131

作者：

Botes, Marelize ^{[1
]}

Loos, Ben ^{[2
]}

van Reenen, Carol A. ^{[1
]}

Dicks, Leon M. T. ^{[1
]}

机构：

[1] Univ Stellenbosch, Dept Microbiol, ZA-7600 Stellenbosch, South Africa

[2] Univ Stellenbosch, Dept Physiol Sci, ZA-7600 Stellenbosch, South Africa

来源：

ARCHIVES OF MICROBIOLOGY | 2008年 / 190卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

adhesion of Enterococcus mundtii; Lactobacillus plantarum to Caco-2 cells;

D O I：

10.1007/s00203-008-0408-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Adhesion of Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 (human carcinoma epithelial) cells was visualized by fluorescent staining. Both strains showed good adhesion compared to L. casei MB1, L. casei Shirota, L. johnsonii La1 and L. rhamnosus GG. No correlation was found between hydrophobicity, aggregation and adhesion to Caco-2 cells. Presence of antibiotics and anti-inflammatory medicaments reduced adhesion of bacterial strains to Caco-2 cells. Proteins sensitive to pepsin, trypsin and pronase are involved in the adhesion of E. mundtii ST4SA and L. plantarum 423 to Caco-2 cells. Adhesion of Listeria monocytogenes ScottA to Caco-2 cells was not prevented by E. mundtii ST4SA and L. plantarum 423. Cell-free culture supernatants of strains ST4SA and 423, containing the antimicrobial peptides plantaricin 423 and peptide ST4SA, prevented the invasion of L. monocytogenes ScottA into Caco-2 cells.

引用

页码：573 / 584

页数：12

共 54 条

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Expression of virulence-related properties by, and intestinal adhesiveness of, Vibrio mimicus strains isolated from aquatic environments [J].