Analysis of Rab1 recruitment to vacuoles containing Legionella pneumophila

被引：12

作者：

Kagan, JC ^{[1
]}

Murata, T ^{[1
]}

Roy, CR ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Sect Microbial Pathogenesis, Boyer Ctr Mol Med, New Haven, CT 06520 USA

来源：

GTPASES REGULATING MEMBRANE TARGETING AND FUSION | 2005年 / 403卷

关键词：

D O I：

10.1016/S0076-6879(05)03007-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Legionella pneumophila is an intracellular bacterial pathogen that utilizes a type IV secretion system to subvert the function of the Rab1 GTPase. Bacterial proteins translocated into host cells mediate the accumulation of the Rab1 protein on vacuoles containing Legionella pneumophila. Assays used to investigate recruitment of Rab1 by L. pneumophila are described. These assays can be used to determine host and bacterial factors required for L. pneumophila subversion of the host secretory pathway.

引用

页码：71 / 81

页数：11

共 17 条

[1] EVIDENCE FOR A GAMMA-INTERFERON RECEPTOR THAT REGULATES MACROPHAGE TUMORICIDAL ACTIVITY [J].

CELADA, A ;

GRAY, PW ;

RINDERKNECHT, E ;

SCHREIBER, RD .

JOURNAL OF EXPERIMENTAL MEDICINE, 1984, 160 (01) :55-74

[2] Legionella effectors that promote nonlytic release from protozoa [J].

Chen, J ;

de Felipe, KS ;

Clarke, M ;

Lu, H ;

Anderson, OR ;

Segal, G ;

Shuman, HA .

SCIENCE, 2004, 303 (5662) :1358-1361

[3] Modulation of phagosome biogenesis by Legionella pneumophila creates an organelle permissive for intracellular growth [J].

Coers, J ;

Monahan, C ;

Roy, CR .

NATURE CELL BIOLOGY, 1999, 1 (07) :451-453

[4] Legionella pneumophila replication vacuole formation involves rapid recruitment of proteins of the early secretory system [J].

Derré, I ;

Isberg, RR .

INFECTION AND IMMUNITY, 2004, 72 (05) :3048-3053

[5]

FEELEY JC, 1979, J CLIN MICROBIOL, V10, P437, DOI 10.1128/JCM.10.4.437-441.1979

[6] FORMATION OF A NOVEL PHAGOSOME BY THE LEGIONNAIRES-DISEASE BACTERIUM (LEGIONELLA-PNEUMOPHILA) IN HUMAN-MONOCYTES [J].

HORWITZ, MA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1983, 158 (04) :1319-1331

[7] THE LEGIONNAIRES-DISEASE BACTERIUM (LEGIONELLA-PNEUMOPHILA) INHIBITS PHAGOSOME-LYSOSOME FUSION IN HUMAN-MONOCYTES [J].

HORWITZ, MA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1983, 158 (06) :2108-2126

[8] TOXOPLASMA-GONDII - FUSION COMPETENCE OF PARASITOPHOROUS VACUOLES IN FC-RECEPTOR TRANSFECTED FIBROBLASTS [J].

JOINER, KA ;

FUHRMAN, SA ;

MIETTINEN, HM ;

KASPER, LH ;

MELLMAN, I .

SCIENCE, 1990, 249 (4969) :641-646

[9] Legionella subvert the functions of Rab1 and Sec22b to create a replicative organelle [J].

Kagan, JC ;

Stein, MP ;

Pypaert, M ;

Roy, CR .

JOURNAL OF EXPERIMENTAL MEDICINE, 2004, 199 (09) :1201-1211

[10] Legionella phagosomes intercept vesicular traffic from endoplasmic reticulum exit sites [J].

Kagan, JC ;

Roy, CR .

NATURE CELL BIOLOGY, 2002, 4 (12) :945-954

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