Salicylic acid interferes with clathrin-mediated endocytic protein trafficking

被引:95
作者
Du, Yunlong [1 ,2 ,3 ]
Tejos, Ricardo [1 ,2 ]
Beck, Martina [4 ]
Himschoot, Ellie [1 ,2 ]
Li, Hongjiang [1 ,2 ]
Robatzek, Silke [4 ]
Vanneste, Steffen [1 ,2 ]
Friml, Jiri [1 ,2 ,5 ]
机构
[1] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium
[2] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium
[3] Yunnan Agr Univ, Educ Minist China, Key Lab Agrobiodivers & Pest Management, Kunming 650201, Peoples R China
[4] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[5] IST Austria, A-3400 Klosterneuburg, Austria
关键词
DEPENDENT ENDOCYTOSIS; DISEASE RESISTANCE; AUXIN TRANSPORTERS; RECEPTOR FLS2; ARABIDOPSIS; EFFLUX; EXPRESSION; DEFENSE; GROWTH; PIN2;
D O I
10.1073/pnas.1220205110
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology.
引用
收藏
页码:7946 / 7951
页数:6
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