Nuclear ribosomal DNA unit length variation: A putative marker of genetic diversity in jojoba

被引:2
作者
Amarger, V
Mercier, L
机构
[1] UFR SCI MED & PHARMACEUT, LAB HISTOL EMBRYOL, F-49045 ANGERS, FRANCE
[2] LRPV, F-49045 ANGERS, FRANCE
关键词
D O I
10.1086/297348
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
jojoba (Simmondsia chinensis [Link] Schneider) is a crop of high economic interest that is grown for the exceptional quality of its seed oil. We have taken advantage of the large variation commonly shown by ribosomal DNA (rDNA) in many plant species to assess the genetic diversity between clones and among individuals in jojoba. The variation of rDNA was analyzed with the use of a homologous 1.7-kbp probe generated by PCR and a 20-mer oligonucleotide probe targeting, respectively, the 17S and 25S coding regions. The multiple-step ladder obtained with the BamH I-EcoR I restriction fragment containing the intergenic spacer (IGS) was the best indicator of variation in length of rDNA unit. In addition to variations observed among selected clones, significant differences in the relative proportion of length variants were seen among individuals grown from seeds of unknown origin.
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页码:296 / 302
页数:7
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