The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus

被引：77

作者：

Majcherczyk, PA ^{[1
]}

McKenna, T

Moreillon, P

Vaudaux, P

机构：

[1] Univ Lausanne, Dept Fundamental Microbiol, CH-1015 Lausanne, Switzerland

[2] Waters Corp, Manchester, Lancs, England

[3] Univ Hosp Geneva, Infect Dis Serv, Geneva, Switzerland

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 255卷 / 02期

关键词：

Staphylococcus aureus; methicillin resistance; teicoplanin resistance; MALDI-TOF mass spectrometry; peptidoglycan muropeptide analysis; pulsed-field gel electrophoresis;

D O I：

10.1111/j.1574-6968.2005.00060.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More important changes in MALDI-TOF MS spectra were found with strains differing in methicillin than in teicoplanin resistance. In comparison, very minor or no changes were recorded in pulsed-field gel electrophoresis profiles or peptidoglycan muropeptide digest patterns of these strains, respectively. MALDI-TOF MS might be useful to detect subtle strain-specific differences in ionizable components released from bacterial surfaces and not from their peptidoglycan network.

引用

页码：233 / 239

页数：7

共 43 条

[1]

Bernardo K, 2002, PROTEOMICS, V2, P747, DOI 10.1002/1615-9861(200206)2:6<747::AID-PROT747>3.0.CO

[2]

2-V

[3] A spectrum of changes occurs in peptidoglycan composition of glycopeptide-intermediate clinical Staphylococcus aureus isolates [J].

Boyle-Vavra, S ;

Labischinski, H ;

Ebert, CC ;

Ehlert, K ;

Daum, RS .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (01) :280-287

[4] Rapid typing of bacteria using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry and pattern recognition software [J].

Bright, JJ ;

Claydon, MA ;

Soufian, M ;

Gordon, DB .

JOURNAL OF MICROBIOLOGICAL METHODS, 2002, 48 (2-3) :127-138

[5] The rapid identification of intact microorganisms using mass spectrometry [J].

Claydon, MA ;

Davey, SN ;

EdwardsJones, V ;

Gordon, DB .

NATURE BIOTECHNOLOGY, 1996, 14 (11) :1584-1586

[6] Contribution of a thickened cell wall and its glutamine nonamidated component to the vancomycin resistance expressed by Staphylococcus aureus Mu50 [J].

Cui, LZ ;

Murakami, H ;

Kuwahara-Arai, K ;

Hanaki, H ;

Hiramatsu, K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (09) :2276-2285

[7] CHARACTERIZATION OF STAPHYLOCOCCUS-AUREUS ISOLATES WITH DECREASED SUSCEPTIBILITY TO VANCOMYCIN AND TEICOPLANIN - ISOLATION AND PURIFICATION OF A CONSTITUTIVELY PRODUCED PROTEIN ASSOCIATED WITH DECREASED SUSCEPTIBILITY [J].

DAUM, RS ;

GUPTA, S ;

SABBAGH, R ;

MILEWSKI, WM .

JOURNAL OF INFECTIOUS DISEASES, 1992, 166 (05) :1066-1072

[8] ALTERED MUROPEPTIDE COMPOSITION IN STAPHYLOCOCCUS-AUREUS STRAINS WITH AN INACTIVATED FEMA LOCUS [J].

DEJONGE, BLM ;

SIDOW, T ;

CHANG, YS ;

LABISCHINSKI, H ;

BERGERBACHI, B ;

GAGE, DA ;

TOMASZ, A .

JOURNAL OF BACTERIOLOGY, 1993, 175 (09) :2779-2782

[9]

DEJONGE BLM, 1992, J BIOL CHEM, V267, P11248

[10] Identification of Staphylococcus aureus and determination of its methicillin resistance by matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry [J].

Du, ZM ;

Yang, RF ;

Guo, ZB ;

Song, YJ ;

Wang, J .

ANALYTICAL CHEMISTRY, 2002, 74 (21) :5487-5491

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