tmRNA-dependent trans-translation is required for sporulation in Bacillus subtilis

被引:25
作者
Abe, Teppei [1 ]
Sakaki, Kazutaka [2 ]
Fujihara, Ai [2 ]
Ujiie, Hiromi [1 ,3 ]
Ushida, Chisato [1 ,4 ]
Himeno, Hyouta [1 ,4 ]
Sato, Tsutomu [5 ]
Muto, Akira [1 ,4 ]
机构
[1] Hirosaki Univ, Fac Agr & Life Sci, Dept Biochem & Mol Biol, Hirosaki, Aomori 0368561, Japan
[2] Hirosaki Univ, Fac Sci, Dept Biol, Hirosaki, Aomori 0368561, Japan
[3] Hirosaki Univ, RNA Res Ctr, Hirosaki, Aomori 0368561, Japan
[4] Iwate Univ, United Grad Sch Agr Sci, Morioka, Iwate 0208551, Japan
[5] Tokyo Univ Agr & Technol, Dept Int Environm Agr Sci, Fuchu, Tokyo, Japan
基金
日本学术振兴会;
关键词
D O I
10.1111/j.1365-2958.2008.06381.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Spore formation in Bacillus subtilis is significantly impaired by the deletion of the gene for tmRNA (ssrA), which facilitates the trans-translation reaction that rescues stalled ribosomes and degrades incompletely synthesized peptides. Microscopic analysis revealed that the sporulation of most Delta ssrA cells is blocked after forespore formation. Expression analysis of lacZ-fused genes directed by several RNA polymerase sigma factors showed that the synthesis of active sigma(K), encoded by the sigK gene, is predominantly inhibited in Delta ssrA cells. The defect in sigma(K) synthesis is attributable to a defect in the skin element excision, which generates the sigK gene, caused in turn by reduced expression of SpoIVCA (recombinase) in Delta ssrA cells.
引用
收藏
页码:1491 / 1498
页数:8
相关论文
共 36 条
[1]   REGULATORY STUDIES ON THE PROMOTER FOR A GENE GOVERNING SYNTHESIS AND ASSEMBLY OF THE SPORE COAT IN BACILLUS-SUBTILIS [J].
CUTTING, S ;
PANZER, S ;
LOSICK, R .
JOURNAL OF MOLECULAR BIOLOGY, 1989, 207 (02) :393-404
[2]   The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis [J].
Eichenberger, P ;
Fujita, M ;
Jensen, ST ;
Conlon, EM ;
Rudner, DZ ;
Wang, ST ;
Ferguson, C ;
Haga, K ;
Sato, T ;
Liu, JS ;
Losick, R .
PLOS BIOLOGY, 2004, 2 (10) :1664-1683
[3]   Regulation of endospore formation in Bacillus subtilis [J].
Errington, J .
NATURE REVIEWS MICROBIOLOGY, 2003, 1 (02) :117-126
[4]   Detection of tmRNA-mediated trans-translation products in Bacillus subtilis [J].
Fujihara, A ;
Tomatsu, H ;
Inagaki, S ;
Tadaki, T ;
Ushida, C ;
Himeno, H ;
Muto, A .
GENES TO CELLS, 2002, 7 (03) :343-350
[5]   TRANSCRIPTION OF SPOIVB IS THE ONLY ROLE OF SIGMA(G) THAT IS ESSENTIAL FOR PRO-SIGMA(K) PROCESSING DURING SPORE FORMATION IN BACILLUS-SUBTILIS [J].
GOMEZ, M ;
CUTTING, S ;
STRAGIER, P .
JOURNAL OF BACTERIOLOGY, 1995, 177 (16) :4825-4827
[6]   The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system [J].
Gottesman, S ;
Roche, E ;
Zhou, YN ;
Sauer, RT .
GENES & DEVELOPMENT, 1998, 12 (09) :1338-1347
[7]   SPORULATION REGULATORY PROTEIN SPOIIID FROM BACILLUS-SUBTILIS ACTIVATES AND REPRESSES TRANSCRIPTION BY BOTH MOTHER-CELL-SPECIFIC FORMS OF RNA-POLYMERASE [J].
HALBERG, R ;
KROOS, L .
JOURNAL OF MOLECULAR BIOLOGY, 1994, 243 (03) :425-436
[8]   Cleavage of the A site mRNA codon during ribosome pausing provides a mechanism for translational quality control [J].
Hayes, CS ;
Sauer, RT .
MOLECULAR CELL, 2003, 12 (04) :903-911
[9]  
HENNER DJ, 1990, METHOD ENZYMOL, V185, P199
[10]   Degradation of carboxy-terminal-tagged cytoplasmic proteins by the Escherichia coli protease HflB (FtsH) [J].
Herman, C ;
Thévenet, D ;
Bouloc, P ;
Walker, GC ;
D'Ari, R .
GENES & DEVELOPMENT, 1998, 12 (09) :1348-1355