In situ detection of diamine oxidase activity using enhanced chemiluminescence

被引：10

作者：

Bruun, L ^{[1
]}

Houen, G ^{[1
]}

机构：

[1] STATENS SERUM INST,DEPT AUTOIMMUNOL,DK-2300 COPENHAGEN,DENMARK

来源：

ANALYTICAL BIOCHEMISTRY | 1996年 / 233卷 / 01期

关键词：

D O I：

10.1006/abio.1996.0017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In need of a simple and sensitive method for detection of diamine oxidase (EC 1.4.3.6) activity in connection with diamine oxidase purification from human placenta, we have developed an enhanced chemiluminescence method using putrescine as substrate and horseradish peroxidase and luminol for the detection of the H2O2 produced by diamine oxidase. The method allows direct detection of small amounts of diamine oxidase in serum samples after agarose gel electrophoresis and allows visualization of diamine oxidase activity in tissue sections, Employing this method we have detected diamine oxidase in sera from cow, horse, monkey, rabbit, and pregnant women. On tissue sections from term human placenta diamine oxidase activity was exclusively localized to the maternal side and was concentrated in vessels and fibrinoid areas. (C) 1996 Academic Press, Inc.

引用

页码：130 / 136

页数：7

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