Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells

被引:67
作者
Jazayeri, Maryam [1 ]
Allameh, Abdolamir [1 ]
Soleimani, Masoud [2 ]
Jazayeri, Seyed Hamid [3 ]
Piryaei, Abbas [4 ]
Kazemnejad, Somaieh [1 ]
机构
[1] Tarbiat Modares Univ, Fac Med, Dept Clin Biochem, Tehran, Iran
[2] Tarbiat Modares Univ, Fac Med Sci, Dept Hematol, Tehran, Iran
[3] NIOC Cent Hosp, Dept Pathol, Tehran, Iran
[4] Shahid Beheshti Univ, Fac Med Sci, Dept Anat, Cell & Mol Biol Res Ctr, Tehran, Iran
关键词
Mesenchymal stem cell; Endothelial cells; Angiogenesis; Differentiation; IGF-1; Weible-Palade body;
D O I
10.1016/j.cellbi.2008.07.020
中图分类号
Q2 [细胞生物学];
学科分类号
071009 [细胞生物学]; 090102 [作物遗传育种];
摘要
In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (IGF-1). A panel of cellular and molecular markers was used for characterization of the endothelial cells. The cells were strongly positive for von Willebrand factor (vWF) and vascular endothelial growth factor receptor 2 (VEGFR2) when measured at protein and mRNA levels. Development of endothelial cells was found to be associated with formation of typical organelles such as Weibel Palade (WP) bodies, Cavealae and pinocytic vesicles. Early vessel growth was also evidenced by showing specific junctions between the cells. The migratory and angiogenic properties of the cells were confirmed by showing capillary network formation in vitro. These results indicate that the capacity of endothelial cells differentiated from hBMSCs in formation of vascular system is consistent with molecular and structural development. (c) 2008 Published by Elsevier Ltd on behalf of International Federation for Cell Biology.
引用
收藏
页码:1183 / 1192
页数:10
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