Deep mutational scanning of an RRM domain of the Saccharomyces cerevisiae poly(A)-binding protein

被引:151
作者
Melamed, Daniel [1 ,2 ]
Young, David L. [2 ]
Gamble, Caitlin E. [2 ]
Miller, Christina R. [1 ,2 ]
Fields, Stanley [1 ,2 ,3 ]
机构
[1] Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA
[2] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA
[3] Univ Washington, Dept Med, Seattle, WA 98195 USA
关键词
epistasis; Pab1; RNA recognition motif; RNA-binding protein; structure; function analysis; RNA RECOGNITION MOTIFS; MESSENGER-RNA; BINDING PROTEINS; EVOLUTIONARY CONSERVATION; GENE-EXPRESSION; SEQUENCE; YEAST; POLY(A); TRANSLATION; NUCLEAR;
D O I
10.1261/rna.040709.113
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The RNA recognition motif (RRM) is the most common RNA-binding domain in eukaryotes. Differences in RRM sequences dictate, in part, both RNA and protein-binding specificities and affinities. We used a deep mutational scanning approach to study the sequence-function relationship of the RRM2 domain of the Saccharomyces cerevisiae poly(A)-binding protein (Pab1). By scoring the activity of more than 100,000 unique Pab1 variants, including 1246 with single amino acid substitutions, we delineated the mutational constraints on each residue. Clustering of residues with similar mutational patterns reveals three major classes, composed principally of RNA-binding residues, of hydrophobic core residues, and of the remaining residues. The first class also includes a highly conserved residue not involved in RNA binding, G150, which can be mutated to destabilize Pab1. A comparison of the mutational sensitivity of yeast Pab1 residues to their evolutionary conservation reveals that most residues tolerate more substitutions than are present in the natural sequences, although other residues that tolerate fewer substitutions may point to specialized functions in yeast. An analysis of similar to 40,000 double mutants indicates a preference for a short distance between two mutations that display an epistatic interaction. As examples of interactions, the mutations N139T, N139S, and I157L suppress other mutations that interfere with RNA binding and protein stability. Overall, this study demonstrates that living cells can be subjected to a single assay to analyze hundreds of thousands of protein variants in parallel.
引用
收藏
页码:1537 / 1551
页数:15
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