Polyphosphate accumulation and oxidative DNA damage in superoxide dismutase-deficient Escherichia coli

被引:12
作者
Al-Maghrebi, MA [1 ]
Benov, LT [1 ]
机构
[1] Kuwait Univ, Fac Med, Dept Biochem, Safat 13110, Kuwait
关键词
polyphosphate; superoxide dismutase-deficient; oxidative DNA damage; hydrogen peroxide; oxidative stress; free radicals;
D O I
10.1016/S0891-5849(01)00696-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Inorganic polyphosphate is a ubiquitous, linear polymer of phosphate residues linked by high-energy phosphoanhydride bonds. In response to starvation, polyP levels are increased up to 100-fold. It has been proposed that chelation of transition metals by polyP might reduce their toxicity, and that polyP accumulation is vital for survival in stationary phase. SOD-deficient E coli is unable to survive in stationary phase. We found that deletion of the cytoplasmic SODs does not impair the cell's capability of synthesizing polyP. However, transient accumulation of polyphosphate correlated with increased resistance to H2O2 and protection of DNA against oxidative damage. The reason for this protective effect of polyP is the induction of HPII catalase and DNA repair enzymes as members of the rpoS regulon. PolyP did not directly protect DNA against oxidative damage in vitro and acted as a pro-oxidant by stimulating the production of hydroxyl radical in the Fenton reaction. It is thus suggested that accumulation of poly P and rpoS induction cannot compensate for the lack of cytosolic SODs. for survival in stationary phase. (C) 2001 Elsevier Science Inc.
引用
收藏
页码:1352 / 1359
页数:8
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