A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

被引：5

作者：

Kao, Chien-Han ^{[3
]}

Cheng, Chiu-Min ^{[4
]}

Chuang, Kuo-Hsiang ^{[5
]}

Chuang, Chih-Hung ^{[6
]}

Tzou, Shey-Cherng ^{[7
]}

Cheng, Ta-Chun ^{[3
]}

Hsieh, Yuan-Chin ^{[3
]}

Liao, Kuang-Wen ^{[7
]}

Wang, Yun-Ming ^{[7
]}

Chang, Long-Sen ^{[8
]}

Roffler, Steve R. ^{[9
]}

Chen, Fang Ming ^{[1
,2
]}

Cheng, Tian-Lu ^{[1
,8
]}

机构：

[1] Kaohsiung Med Univ, Dept Biomed Sci & Environm Biol, Kaohsiung, Taiwan

[2] Kaohsiung Med Univ, Dept Surg, Fac Med, Coll Med, Kaohsiung, Taiwan

[3] Kaohsiung Med Univ, Grad Inst Med, Kaohsiung, Taiwan

[4] Natl Kaohsiung Marine Univ, Dept Aquaculture, Kaohsiung, Taiwan

[5] Taipei Med Univ, Grad Inst Pharmacognosy, Taipei, Taiwan

[6] Natl Cheng Kung Univ, Inst Basic Med Sci, Tainan 70101, Taiwan

[7] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu, Taiwan

[8] Natl Sun Yat Sen Univ, Inst Biomed Sci, Kaohsiung 80424, Taiwan

[9] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 431卷 / 01期

关键词：

14-Epitope tag protein ladder; Epitope tags; Anti-epitope tag antibodies; Protein molecular estimation; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Western blot analysis; MOLECULAR-WEIGHT MARKERS; GEL-ELECTROPHORESIS; FUSION PROTEINS; CALIBRATION; STANDARDS; PCR;

D O I：

10.1016/j.ab.2012.08.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We designed a protein ladder (hereafter referred to as "Mega-tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R-2 = 0.997 +/- 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：1 / 3

页数：3

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