Identification of a molluscan homologue of the neuroendocrine polypeptide 7B2

被引:17
作者
Spijker, S
Smit, AB
Martens, GJM
Geraerts, WPM
机构
[1] CATHOLIC UNIV NIJMEGEN, DEPT ANIM PHYSIOL, NL-6525 ED NIJMEGEN, NETHERLANDS
[2] FREE UNIV AMSTERDAM, NEUROSCI RES INST, GRAD SCH NEUROSCI AMSTERDAM, NL-1081 HV AMSTERDAM, NETHERLANDS
关键词
D O I
10.1074/jbc.272.7.4116
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In vertebrates, interaction of prohormone convertase 2 (PC2) with the highly conserved polypeptide 7B2 is essential for transport and maturation of proPC2 in the regulated secretory pathway. In vitro, 7B2 displays a strong inhibitory activity toward PC2, Here, we characterize a cDNA encoding the first invertebrate 7B2-related protein (L7B2) from the brain of the mollusc Lymnaea stagnalis. The overall amino acid sequence identity between L7B2 and its vertebrate counterparts is surprisingly low (29%) and is restricted to a few small stretches of amino acid residues, Of particular interest are a conserved proline-rich region in the middle portion of the L7B2 sequence and a repeated conserved region in the carboxyl-terminal domain. Synthetic peptides corresponding to the carboxyl-terminal regions inhibit Lymnaea PC2 enzyme activity in extracts of insulin-producing neurons, in which both L7B2 and Lymnaea PC2 are abundantly expressed. Moreover, the peptides inhibit mouse PC2 enzyme activity. Our cloning of invertebrate 7B2 helps to delineate residues that are important for 7B2-PC2 interaction.
引用
收藏
页码:4116 / 4120
页数:5
相关论文
共 24 条
[1]  
AYOUBI TAY, 1990, J BIOL CHEM, V265, P15644
[2]   7B2 IS A NEUROENDOCRINE CHAPERONE THAT TRANSIENTLY INTERACTS WITH PROHORMONE CONVERTASE PC2 IN THE SECRETORY PATHWAY [J].
BRAKS, JAM ;
MARTENS, GJM .
CELL, 1994, 78 (02) :263-273
[3]   Purification and enzymatic characterization of recombinant prohormone convertase 2: Stabilization of activity by 21 kDa 7B2 [J].
Lamango, NS ;
Zhu, XR ;
Lindberg, I .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1996, 330 (02) :238-250
[4]  
LAZURE C, 1991, INT J PEPT PROT RES, V38, P392
[5]   ENZYMATIC CHARACTERIZATION OF IMMUNOPURIFIED PROHORMONE CONVERTASE .2. POTENT INHIBITION BY A 7B2 PEPTIDE FRAGMENT [J].
LINDBERG, I ;
VANDENHURK, WH ;
BUI, C ;
BATIE, CJ .
BIOCHEMISTRY, 1995, 34 (16) :5486-5493
[6]   CLONING AND SEQUENCE-ANALYSIS OF HUMAN PITUITARY CDNA-ENCODING THE NOVEL POLYPEPTIDE 7B2 [J].
MARTENS, GJM .
FEBS LETTERS, 1988, 234 (01) :160-164
[7]   THE NEUROENDOCRINE POLYPEPTIDE 7B2 IS AN ENDOGENOUS INHIBITOR OF PROHORMONE CONVERTASE PC2 [J].
MARTENS, GJM ;
BRAKS, JAM ;
EIB, DW ;
ZHOU, Y ;
LINDBERG, I .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :5784-5787
[8]   THE NOVEL PITUITARY POLYPEPTIDE 7B2 IS A HIGHLY-CONSERVED PROTEIN COEXPRESSED WITH PROOPIOMELANOCORTIN [J].
MARTENS, GJM ;
BUSSEMAKERS, MJG ;
AYOUBI, TAY ;
JENKS, BG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 181 (01) :75-79
[9]   DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].
SANGER, F ;
NICKLEN, S ;
COULSON, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467
[10]  
SCHAFER MKH, 1993, J NEUROSCI, V13, P1258