Shoot regeneration in four Begonia genotypes

被引:27
作者
Espino, FJ [1 ]
Linacero, R [1 ]
Rueda, J [1 ]
Vázquez, AM [1 ]
机构
[1] Univ Complutense Madrid, Fac Biol, Dept Genet, E-28040 Madrid, Spain
关键词
explant type; genotype effect; growth regulators; in vitro culture; organogenic response;
D O I
10.1023/B:BIOP.0000024282.01087.a3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In vitro regeneration of four Begonia genotypes, B. semperflorens, B. rex, B. x elatior, and hybrid of Begonia with unknown parents 'Tiger' was carried out starting from leaf and petiole segments as explants. Five Murashige and Skoog's derived media were tested, three of them supplemented with alpha-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), and the other two with NAA and kinetin (KIN) in different concentrations. Shoot regeneration was preferentially induced on the BA containing media, quantitative differences being observed among explants and genotypes.
引用
收藏
页码:101 / 104
页数:4
相关论文
共 11 条
[1]   Micropropagation of Cardiospermum halicacabum [J].
Babber, S ;
Mittal, K ;
Ahlawat, R ;
Varghese, TM .
BIOLOGIA PLANTARUM, 2001, 44 (04) :603-606
[2]   Organogenesis in cultured petiole explants of Begonia x erythrophylla: The timing and specificity of the inductive stimuli [J].
Burritt, DJ ;
Leung, DWM .
JOURNAL OF EXPERIMENTAL BOTANY, 1996, 47 (297) :557-567
[3]   VARIATION IN ADVENTITIOUS REGENERANTS OF BEGONIA-REX PUTZ LUCILLE CLOSON AS A CONSEQUENCE OF CELL ONTOGENY, CALLUS AGING AND FREQUENCY OF CALLUS SUBCULTURE [J].
CASSELLS, AC ;
MORRISH, FM .
SCIENTIA HORTICULTURAE, 1987, 32 (1-2) :135-143
[4]   DIFFERENTIAL REACTIVITY IN EPIDERMAL-CELLS OF BEGONIA-REX EXCISED AND GROWN INVITRO [J].
CHLYAH, A ;
TRANTHANHVAN, M .
PHYSIOLOGIA PLANTARUM, 1975, 35 (01) :16-20
[5]  
KHODER M, 1981, CR ACAD SCI III-VIE, V293, P403
[6]   Agrobacterium tumefaciens-mediated transformation of Elatior Begonia (Begonia x hiemalis Fotsch) [J].
Kishimoto, S ;
Aida, R ;
Shibata, M .
PLANT SCIENCE, 2002, 162 (05) :697-703
[7]  
Kiyokawa S, 1996, PLANT CELL REP, V15, P606, DOI 10.1007/BF00232462
[8]   A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].
MURASHIGE, T ;
SKOOG, F .
PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497
[9]   Micropropagation of Spilanthes acmella Murr. [J].
Saritha, KV ;
Prakash, E ;
Ramamurthy, N ;
Naidu, CV .
BIOLOGIA PLANTARUM, 2002, 45 (04) :581-584