RNAi mediated MMP-1 silencing inhibits human chondrosarcoma invasion

被引：86

作者：

Yuan, J

Dutton, CM

Scully, SP

机构：

[1] Univ Miami, Dept Orthopaed & Rehabil, Miami, FL 33101 USA

[2] Mayo Clin, Dept Orthopaed, Rochester, MN USA

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2005年 / 23卷 / 06期

关键词：

chondrosarcoma; MMP-1; siRNA; metastasis;

D O I：

10.1016/j.orthres.2005.04.004

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Previous work indicates that matrix metalloprotemase-1 (MMP-1) gene expression is a prognostic factor for local recurrence and metastasis in human chondrosarcoma. The current study evaluates the effects of MMP-1 knock-down via RNA interference on chondrosarcoma metastasis in vitro. Of nine siRNA oligos tested, one showed a 90% inhibitory effect on MMP-1 gene expression. Stable attenuation of MMP-1 in chondrosarcoma cells was achieved by using a plastuid vector-based siRNA system. Pronounced MMP-1 activity suppression was accompanied by marked inhibition of tumor cell invasion. Cells in which MMP-1 has been stably silenced are useful tools to elucidate the mechanism of MMP-1 mediated tumor metastasis. (c) 2005 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：1467 / 1474

页数：8

共 36 条

[1] A novel host/tumor cell interaction activates matrix metalloproteinase 1 and mediates invasion through type I collagen [J].