Astrocyte-conditioned medium induces blood-brain barrier properties in endothelial cells

被引：26

作者：

Yamagata, K

Tagami, M

Nara, Y

Mitani, M

Kubota, A

Fujino, H

Numano, F

Kato, T

Yamori, Y

机构：

[1] SUMITOMO MET IND LTD,DIV BIOMED,TOKYO,JAPAN

[2] SANRAKU HOSP,DEPT MED,TOKYO,JAPAN

[3] TOKYO MED & DENT UNIV,DEPT MED 3,TOKYO 113,JAPAN

[4] NAGOYA CITY UNIV,SCH MED,DEPT BIOREGULAT RES,NAGOYA,AICHI 467,JAPAN

[5] KYOTO UNIV,GRAD SCH HUMAN & ENVIRONM STUDIES,KYOTO 606,JAPAN

来源：

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | 1997年 / 24卷 / 9-10期

关键词：

astrocyte; blood-brain barrier; endothelial cells; extracellular matrix; proteoglycan; tight junction; ultrastructure;

D O I：

10.1111/j.1440-1681.1997.tb02117.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte-derived supernatant in culture using horseradish peroxidase (HRP) as a parameter, 2, A gel filtration fraction (molecular weight 16-20 kDa) of type 1 astrocyte-derived supernatant reduced plasmalemmal vesicles and induced tight junctions in endothelial cells, 3, In addition, this fraction promoted the uptake of [H-3]-glucosamine by endothelial cells, This cell activity disappeared after heating at 95 degrees C for 10 min and was sensitive to trypsin, 4, These results suggest that a type 1 astrocyte-derived protein factor(s) promotes proteoglycan synthesis with a resultant increase in charge selectivity in endothelial cells and plays an important role in the induction of blood-brain barrier functions.

引用

页码：710 / 713

页数：4

共 22 条

[1] ASTROCYTE-MEDIATED INDUCTION OF TIGHT JUNCTIONS IN BRAIN CAPILLARY ENDOTHELIUM - AN EFFICIENT INVITRO MODEL [J].