Transforming growth factor-β1 mediates the effects of low-intensity pulsed ultrasound in chondrocytes

被引：123

作者：

Mukai, S ^{[1
]}

Ito, H ^{[1
]}

Nakagawa, Y ^{[1
]}

Akiyama, H ^{[1
]}

Miyamoto, M ^{[1
]}

Nakamura, T ^{[1
]}

机构：

[1] Kyoto Univ, Dept Orthopaed Surg, Kyoto 6068507, Japan

来源：

ULTRASOUND IN MEDICINE AND BIOLOGY | 2005年 / 31卷 / 12期

关键词：

low-intensity pulsed ultrasound; aggregate culture; chondrocyte differentiation; transforming growth factor-beta(1); anti-TGF-beta(1); neutralizing antibody;

D O I：

10.1016/j.ultrasmedbio.2005.07.012

中图分类号：

O42 [声学];

学科分类号：

070206 [声学]; 082403 [水声工程];

摘要：

Low-intensity pulsed ultrasound (LIPUS) has been shown to accelerate fracture healing, but the precise mechanism is still unknown. We used aggregate chondrocyte culture system to analyze LIPUS-induced effects on chondrocytes. First, Northern analyses revealed that LIPUS maintained higher expression levels of type II collagen and aggrecan mRNA and delayed the appearance of type X collagen mRNA expression. We also showed that DNA content was increased and that alkaline phosphatase activity was maintained low by daily treatment. Moreover, LIPUS significantly promoted transforming growth factor (TGF)-beta(1) mRNA expression and the protein production at 2 h and 12 h after the treatment, respectively. Furthermore, recombinant TGF-beta(1) protein mimicked the LIPUS effect and anti-TGF-beta(1) neutralizing antibody reversed all these changes induced by the LIPUS treatment. These results indicate that LIPUS promotes the proliferation and retains the differentiation state of chondrocytes in the aggregate culture and that TGF-beta(1) plays an important role in mediating the LIPUS effects in chondrocytes. (E-mail: smukai@kyotolan.hosp.go.jp) (c) 2005 World Federation for Ultrasound in Medicine & Biology.

引用

页码：1713 / 1721

页数：9

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