Peripheral injection of lipopolysaccharide prevents brain recruitment of leukocytes induced by central injection of interleukin-1

I.c.v. injection of interieukin-1 beta induces infiltration of leukocytes into the brain. I.p. injection of bacterial endotoxin lipopolysaccharlde induces the expression of interieukin-1 in the CNS without causing the entry of leukocytes into the brain. This suggests that during systemic inflammation trafficking of potentially damaging leukocytes into the CNS is inhibited. In this study, we investigated the effects of peripheral injection of lipopolysaccharide on brain leukocyte recruitment induced by i.c.v.-interieukin-1 in mice. I.c.v.-interieukin-I induced widespread infiltration of leukocytes into the brain 16 h after the injection. Pretreatment with i.p.-lipopolysaccharide 2 h before the i.c.v. interieukin-1 injection completely blocked interleukin1-induced leukocyte infiltration, whereas i.p.-LPS only attenuated the effect of interleukin-1 if it was given 12 h before i.c.v. interleukin-1 injection. I.p lipopolysaccharide given 24 h before i.c.v. interieukin-1 injection did not alter interieukin-1 induced leukocyte infiltration. i.c.v.-interleukin-1 induced expression of p- and e-selectins in brain vasculatures prior to the appearance of leukocytes in the brain parenchyma. Induction of p- and e-selectin was inhibited by the pretreatment of i.p.-lipopolysaccharlde 2 h, but not 24 h, before i.c.v.-interleukin-1 injection. i.c.v.-interieukin-1-induced leukocyte infiltration was diminished in both e- and p- selectin knockout animals. These results suggest that systemic inflammation actively inhibits recruitment of leukocytes by CNS. Inhibition of the expression of p- and e-selectins is a mechanism by which peripheral inflammation regulate CNS leukocyte recruitment. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.